ArrayExpress Uploader08526bone marrowPurified Pro-B (B220-positive/CD43-high/IgM-negative) B-cells129Wild-typebone marrowPurified Pro-B (B220-positive/CD43-high/IgM-negative) B-cells129Wild-typebone marrowPurified Pro-B (B220-positive/CD43-high/IgM-negative) B-cellsB6Wild-typebone marrowPurified Pre-B (B220-positive/CD43-low/IgM-negative) B-cells129Wild-typebone marrowPurified Pre-B (B220-positive/CD43-low/IgM-negative) B-cells129Wild-typebone marrowPurified Pre-B (B220-positive/CD43-low/IgM-negative) B-cells129Wild-typespleenPurified Transitional (B220-positive/Cd93-positive) B-cells129Wild-typespleenPurified Transitional (B220-positive/Cd93-positive) B-cells129Wild-typespleenPurified Transitional (B220-positive/Cd93-positive) B-cells129Wild-typespleenPurified Transitional (B220-positive/Cd93-positive) B-cells129Wild-typespleenPurified Follicular (Cd19-positive/Cd21-intermediate/Cd23-high) B-cells129Wild-typespleenPurified Follicular (Cd19-positive/Cd21-intermediate/Cd23-high) B-cells129Wild-typespleenPurified Follicular (Cd19-positive/Cd21-intermediate/Cd23-high) B-cells129Wild-typespleenPurified Marginal Zone (Cd19-positive/Cd21-high/Cd23-low) B-cells129Wild-typespleenPurified Marginal Zone (Cd19-positive/Cd21-high/Cd23-low) B-cells129Wild-typespleenPurified Marginal Zone (Cd19-positive/Cd21-high/Cd23-low) B-cells129Wild-typespleenPurified Germinal Centre (B220-positive/PNA-high) B-cellsB6Wild-typespleenPurified Germinal Centre (B220-positive/PNA-high) B-cellsB6Wild-typespleenPurified Germinal Centre (B220-positive/PNA-high) B-cellsB6Wild-typespleenPurified Germinal Centre (B220-positive/PNA-high) B-cellsB6Wild-typespleenPurified Germinal Centre (B220-positive/PNA-high) B-cellsB6Wild-typespleenPurified Plasmablasts (B220-positive/Cd138-positive/Cd93-negative)B6Wild-typespleenPurified Plasmablasts (B220-positive/Cd138-positive/Cd93-negative)B6Wild-typespleenPurified Plasmablasts (B220-positive/Cd138-positive/Cd93-negative)B6Wild-typespleenPurified Plasmablasts (B220-positive/Cd138-positive/Cd93-negative)B6Wild-typebone marrowPurified Plasma cells (B220-low/Cd138-positive/Cd93-negative)B6Wild-typebone marrowPurified Plasma cells (B220-low/Cd138-positive/Cd93-negative)B6Wild-typebone marrowPurified Plasma cells (B220-low/Cd138-positive/Cd93-negative)B6Wild-typeTumorLymphoid tumorBcl6 transgenic (Catoretti et al., Cancer Cell 2005Bcl6 knock-inTumorLymphoid tumorBcl6 transgenic (Catoretti et al., Cancer Cell 2005Bcl6 knock-inTumorLymphoid tumorBcl6 transgenic (Catoretti et al., Cancer Cell 2005Bcl6 knock-inTumorLymphoid tumorBcl6 transgenic (Catoretti et al., Cancer Cell 2005Bcl6 knock-inTumorLymphoid tumorMyc/Bcl6 dual-transgenic (Pasqualucci et al., Nat. Genet. 2008;40:108-112)Bcl6/Myc dual-transgenicTumorLymphoid tumorMyc/Bcl6 dual-transgenic (Pasqualucci et al., Nat. Genet. 2008;40:108-112)Bcl6/Myc dual-transgenicTumorLymphoid tumorMyc/Bcl6 dual-transgenic (Pasqualucci et al., Nat. Genet. 2008;40:108-112)Bcl6/Myc dual-transgenicTumorLymphoid tumorLig4/P53 KO (Frank et al., Mol. Cell 2000;5:993-1002)Lig4/p53 double knock-outTumorLymphoid tumorLig4/P53 KO (Frank et al., Mol. Cell 2000;5:993-1002)Lig4/p53 double knock-outTumorLymphoid tumorLig4/P53 KO (Frank et al., Mol. Cell 2000;5:993-1002)Lig4/p53 double knock-outTumorLymphoid tumorLig4/P53 KO (Frank et al., Mol. Cell 2000;5:993-1002)Lig4/p53 double knock-outTumorLymphoid tumorLig4/P53 KO (Frank et al., Mol. Cell 2000;5:993-1002)Lig4/p53 double knock-outTumorLymphoid tumorLig4/P53 KO (Frank et al., Mol. Cell 2000;5:993-1002)Lig4/p53 double knock-outarrayexpress_sid8Heirarchical development of B-cells involves the induction and supression of large sets of genes that provide the basis for...21282644E-GEOD-26408/profile/8773/arrayexpressuploader441bonebone marrowcelllymphomaspleenDec.12, 2014Falsesignatures-of-murine-b-cell-development-implicateE-GEOD-26408_A-AFFY-36Signatures of murine B-cell development implicate Yy1 as a regulator of the germinal center-specific programNov.24, 2014Heirarchical development of B-cells involves the induction and supression of large sets of genes that provide the basis for differentiation and, ultimately, antibody production. We used microarrays to define comprehensive differentiation state-specific transcriptional signatures in order to gain insight into B-cell biology. In addition, we compared profiles of normal B-cells to those of murine lymphoma models that are putatively aligned with different states. B-cells of different differentiation states were purified from the bone marrow and spleen of mice use flow cytometry with antibodies specific for pre-defined markers of each differentiation state. Purified populations were then profiled using Affymetrix Mouse 430A2 gene expression microarrays. Tumors from Bcl6-trangenic mice, Bcl6/Myc dual-transgenic mice and p53/Lig4 double knock-out mice were also profiled using these microarrays in order to assess the similarities/differences between normal and malignant B-cells.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26408mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26408/samples/