{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM647150 1": {}}, {"GSM647151 1": {}}, {"GSM647152 1": {}}, {"GSM647153 1": {}}, {"GSM647154 1": {}}, {"GSM647155 1": {}}, {"GSM647156 1": {}}, {"GSM647157 1": {}}, {"GSM647158 1": {}}], "id": 3905, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Analysis of mobilized peripheral blood CD34+ cells from a healthy volunteer under erythroid differentiation conditions with and without...", "geo_gse_id": "E-GEOD-26351", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 9, "tags": ["peripheral"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-for-mobilized-peripheral-blood-cd3", "geo_id_plat": "E-GEOD-26351_A-AFFY-44", "name": "Expression data for Mobilized Peripheral Blood CD34+ cells with Wnt or BMP stimulation", "created": "Sep.15, 2014", "summary": "Analysis of mobilized peripheral blood CD34+ cells from a healthy volunteer under erythroid differentiation conditions with and without stimulation to the BMP or Wnt signaling pathways. For erythroid differentiation, expanded CD34+ cells were placed in Stemspan SFEM medium supplemented with 2% pen/strep, 20ng/ml SCF, 1U/ml Epo, 5ng/ml IL3, 2uM dexamethasone, and 1uM beta-estradiol. Arrays were performed 2 hours after addition of cytokines. For signaling pathway stimulation, cells were exposed to 0.5uM BIO (a GSK3 inhibitor) for Wnt pathway activation, 25ng/ml rhBMP4 for BMP pathway activation, or vehicle control for 2 hours. Three biological replicates were performed per treatment group. We used microarrays to detail the global program of gene expression changes after Wnt or BMP pathway stimulation in human CD34+ hematopoietic progenitors under erythroid differentiation conditions. To investigate the changes to gene expression in human CD34+ hematopoietic progenitors following stimulation of the Wnt or BMP pathways during the early stages of erythroid differentiation. Three biological replicates were performed per treatment group.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26351", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26351/samples/"}