ArrayExpress Uploader0human3905arrayexpress_sid4Analysis of mobilized peripheral blood CD34+ cells from a healthy volunteer under erythroid differentiation conditions with and without...E-GEOD-26351/profile/8773/arrayexpressuploader09peripheralDec.12, 2014Falseexpression-data-for-mobilized-peripheral-blood-cd3E-GEOD-26351_A-AFFY-44Expression data for Mobilized Peripheral Blood CD34+ cells with Wnt or BMP stimulationSep.15, 2014Analysis of mobilized peripheral blood CD34+ cells from a healthy volunteer under erythroid differentiation conditions with and without stimulation to the BMP or Wnt signaling pathways. For erythroid differentiation, expanded CD34+ cells were placed in Stemspan SFEM medium supplemented with 2% pen/strep, 20ng/ml SCF, 1U/ml Epo, 5ng/ml IL3, 2uM dexamethasone, and 1uM beta-estradiol. Arrays were performed 2 hours after addition of cytokines. For signaling pathway stimulation, cells were exposed to 0.5uM BIO (a GSK3 inhibitor) for Wnt pathway activation, 25ng/ml rhBMP4 for BMP pathway activation, or vehicle control for 2 hours. Three biological replicates were performed per treatment group. We used microarrays to detail the global program of gene expression changes after Wnt or BMP pathway stimulation in human CD34+ hematopoietic progenitors under erythroid differentiation conditions. To investigate the changes to gene expression in human CD34+ hematopoietic progenitors following stimulation of the Wnt or BMP pathways during the early stages of erythroid differentiation. Three biological replicates were performed per treatment group.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26351http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26351/samples/