{"platform": 4, "owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM645710 1": {}}, {"GSM645711 1": {}}, {"GSM645712 1": {}}, {"GSM645713 1": {}}, {"GSM645714 1": {}}, {"GSM645715 1": {}}, {"GSM645716 1": {}}, {"GSM645717 1": {}}, {"GSM645718 1": {}}, {"GSM645719 1": {}}, {"GSM645720 1": {}}, {"GSM645721 1": {}}], "id": 3901, "pop_total": 0, "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26298", "summary_wrapped": "Under conditions of hormonal adjuvant treatment the estrogen receptor apoprotein supports breast cancer cell cycling through the retinoic...", "pubmed_id": 21299862, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 12, "tags": ["basal", "breast", "breast cancer", "cancer", "cell", "estrogen", "glucose", "glutamine", "hormone"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "under-conditions-of-hormonal-adjuvant-treatment-th", "geo_id_plat": "E-GEOD-26298_A-AFFY-44", "name": "Under conditions of hormonal adjuvant treatment the estrogen receptor apoprotein supports breast cancer cell cycling through the retinoic acid receptor \u03b11 apoprotein", "created": "Sep.15, 2014", "summary": "Under conditions of hormonal adjuvant treatment the estrogen receptor apoprotein supports breast cancer cell cycling through the retinoic acid receptor \u03b11 apoprotein. Basal proliferation persisted in estrogen-sensitive breast cancer cells grown in hormone depleted conditioned media without or with 4-hydroxytamoxifen (OH-Tam). Downregulating ER using siRNA  inhibited basal proliferation by promoting cell cycle arrest. The basal expression of RAR\u03b11, the only RAR\u03b1 isoform that was expressed in breast cancer cell lines and in most breast tumors, was supported by apo-ER but was unaffected by OH-Tam. The overlapping tamoxifen-insensitive gene regulation by apo-ER and apo-RAR\u03b11 comprised activation of mainly genes promoting cell cycle and mitosis and suppression of genes involved in growth inhibition. Cells were plated at 20% confluence in low glucose phenol red free medium supplemented with 5% charcoal stripped FBS and glutamine 24h-48h prior to transfection. Treatment with vehicle, OH-Tam (100nM), or OH-Tam (500nM) was begun an additional 24h later. Cells were transfected with control siRNA, ER\u03b1 siRNA or RAR\u03b1 siRNA.", "geo_gse_id": "E-GEOD-26298", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26298/samples/"}