{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM638914": {"GENOTYPE": "wild type", "TREATMENT": "control"}}, {"GSM638914": {"GENOTYPE": "wild type", "TREATMENT": "control"}}, {"GSM638914": {"GENOTYPE": "wild type", "TREATMENT": "control"}}, {"GSM638917": {"GENOTYPE": "wild type", "TREATMENT": "prenatal stress"}}, {"GSM638917": {"GENOTYPE": "wild type", "TREATMENT": "prenatal stress"}}, {"GSM638917": {"GENOTYPE": "wild type", "TREATMENT": "prenatal stress"}}, {"GSM638920": {"GENOTYPE": "5HTT +/-", "TREATMENT": "control"}}, {"GSM638920": {"GENOTYPE": "5HTT +/-", "TREATMENT": "control"}}, {"GSM638920": {"GENOTYPE": "5HTT +/-", "TREATMENT": "control"}}, {"GSM638923": {"GENOTYPE": "5HTT +/-", "TREATMENT": "prenatal stress"}}, {"GSM638923": {"GENOTYPE": "5HTT +/-", "TREATMENT": "prenatal stress"}}, {"GSM638923": {"GENOTYPE": "5HTT +/-", "TREATMENT": "prenatal stress"}}], "id": 6302, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "prenatal stress response, genetic modification Background: Prenatal stress (PS) exposure has been shown to increase the risk for...", "geo_gse_id": "E-GEOD-26025", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["cytokine", "genome", "hippocampus", "serotonin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "sex-specific-effects-of-prenatal-stress-in-5-htt-d", "geo_id_plat": "E-GEOD-26025_A-AFFY-45", "name": "Sex-specific effects of prenatal stress in 5-Htt deficient mice: towards molecular mechanisms of gene x environment interactions", "created": "Nov.11, 2014", "summary": "prenatal stress response, genetic modification Background: Prenatal stress (PS) exposure has been shown to increase the risk for emotional disorders in later life. Furthermore, the serotonin transporter (5-HTT) genotype is suggested to exert a modulating effect on the association between early life stress and the risk for depression. In the present study, we use a 5-HTT x PS paradigm to investigate whether the effects of PS are dependent upon the 5-HTT genotype.  Methods: The effects of PS on cognition, anxiety- and depression-related behaviour were examined using a maternal restraint stress paradigm of PS in C57BL6 wild-type (WT) and heterozygous (+/-) 5-HTT knockout mice. Additionally, in the female offspring, a genome-wide hippocampal gene expression screening was performed.  Results: 5-HTT +/- offspring showed enhanced memory performance and signs of reduced anxiety as compared to WT offspring. Conversely, exposure of 5-HTT +/- mice to PS was associated with altered stress-responsivity and increased depressive-like behaviour, particularly in female offspring. Further, 5-HTT genotype, PS and their interaction differentially affected the expression of numerous genes and related pathways within the female hippocampus. Specifically, MAPK and neurotrophin signalling were regulated by both the 5-HTT +/- genotype and PS exposure, whereas cytokine and Wnt signalling were affected in a 5-HTT genotype x PS manner, indicating a gene x environment interaction at the molecular level.  Conclusions: The long-term behavioural effects of PS in C57BL6 mice are partly dependent on the 5-HTT genotype. Further, hippocampal gene expression profiles suggest that distinct molecular mechanisms mediate the behavioural effects of the 5-HTT genotype, PS exposure, and their interaction. total samples analysed are 12", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26025", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-26025/samples/"}