ArrayExpress Uploaderarrayexpress_sid62963 daily administrations of IL-25.0 days in culture3 daily administrations of IL-25.0 days in culture3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.9 days in culture3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.9 days in culture3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.9 days in culture06Nuocytes are a recently described cell that responds to both IL-25 and IL-33 and produce high levels of IL-13 and IL-5 Mice were...20200518E-GEOD-25890/profile/8773/arrayexpressuploader25cellspleenDec.12, 2014FalseE-GEOD-25890_A-AFFY-45expression-data-from-mouse-nuocytesExpression data from mouse NuocytesNov.11, 2014Nuocytes are a recently described cell that responds to both IL-25 and IL-33 and produce high levels of IL-13 and IL-5 Mice were administered with 3 daily doses of IL-25 (400 ng/dose). Nuocytes were purified by flow cytometry. Spleen cells prepared from IL-25-treated mice were depleted of lineage+ cells before cell sorting by incubation with biotin-conjugated anti-CD3, anti-CD19, anti-CD11b and anti-FcεRI antibodies before removal of antibody-bound cells by magnetic separation using Dynabeads (Invitrogen). Lineage-depleted cells were stained with PE-conjugated antibodies against CD4, CD8, B220, TER-119 and CD11b, a FITC-conjugated antibody against CD45, and an APC-conjugated antibody against ICOS. PE−, FITC+, APC+ cells were collected using a Mo-flo cell sorter, and purity was checked by staining with lineage antibodies and antibodies against IL-17BR and T1/ST2. Following purification nuocytes were prepared directly for microarray gene expression analysis or cultured with IL-33 and IL-7 (both at 10 nanograms/ml) for 9 days before being prepared for microarray gene expression analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25890mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25890/samples/