{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 6296, "factors": [{"GSM635583": {"TRREATMENT": "3 daily administrations of IL-25.", "TIME": "0 days in culture"}}, {"GSM635583": {"TRREATMENT": "3 daily administrations of IL-25.", "TIME": "0 days in culture"}}, {"GSM635585": {"TRREATMENT": "3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.", "TIME": "9 days in culture"}}, {"GSM635585": {"TRREATMENT": "3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.", "TIME": "9 days in culture"}}, {"GSM635585": {"TRREATMENT": "3 daily administrations of IL-25 and cultured with IL-33 and IL-7 (both at 10 nanograms /ml) for 9 days.", "TIME": "9 days in culture"}}], "pop_total": 0, "platform": 6, "summary_wrapped": "Nuocytes are a recently described cell that responds to both IL-25 and IL-33 and produce high levels of IL-13 and IL-5 Mice were...", "pubmed_id": 20200518, "geo_gse_id": "E-GEOD-25890", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 5, "tags": ["cell", "spleen"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-25890_A-AFFY-45", "slug": "expression-data-from-mouse-nuocytes", "geo_gds_id": "", "name": "Expression data from mouse Nuocytes", "created": "Nov.11, 2014", "summary": "Nuocytes are a recently described cell that responds to both IL-25 and IL-33 and produce high levels of IL-13 and IL-5 Mice were administered with 3 daily doses of IL-25 (400 ng/dose).  Nuocytes were purified by flow cytometry. Spleen cells prepared from IL-25-treated mice were depleted of lineage+ cells before cell sorting by incubation with biotin-conjugated anti-CD3, anti-CD19, anti-CD11b and anti-Fc\u03b5RI antibodies before removal of antibody-bound cells by magnetic separation using Dynabeads (Invitrogen). Lineage-depleted cells were stained with PE-conjugated antibodies against CD4, CD8, B220, TER-119 and CD11b, a FITC-conjugated antibody against CD45, and an APC-conjugated antibody against ICOS. PE\u2212, FITC+, APC+ cells were collected using a Mo-flo cell sorter, and purity was checked by staining with lineage antibodies and antibodies against IL-17BR and T1/ST2.  Following purification nuocytes were prepared directly for microarray gene expression analysis or cultured with IL-33 and IL-7 (both at 10 nanograms/ml) for 9 days before being prepared for microarray gene expression analysis.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25890", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25890/samples/"}