{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM635236": {"CELL TYPE": "undifferentiated Oct4, Sox2, cMyc, Klf4 doxycycline inducible mouse trophoblast stem cells"}}, {"GSM635237": {"CELL TYPE": "trophoblast stem cell derived induced pluripotent stem cells line2, clone1"}}, {"GSM635238": {"CELL TYPE": "trophoblast stem cell derived induced pluripotent stem cells line2, clone2"}}, {"GSM635239": {"CELL TYPE": "undifferentiated mouse embryonic stem cells"}}], "id": 6294, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Extraembryonic trophoblast stem cells (TSC) can be converted to induced pluripotent stem cells (TSC-iPSCs) by overexpressing Oct4, Sox2,...", "geo_gse_id": "E-GEOD-25872", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["cell", "fibroblast", "stem cell", "trophoblast"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-25872_A-AFFY-45", "slug": "conversion-of-murine-extraembryonic-trophoblast-st", "geo_gds_id": "", "name": "Conversion of murine extraembryonic trophoblast stem cells into induced pluripotent stem cells", "created": "Nov.11, 2014", "summary": "Extraembryonic trophoblast stem cells (TSC) can be converted to induced pluripotent stem cells (TSC-iPSCs) by overexpressing Oct4, Sox2, Klf4 and cMyc. TSC lines were derived from mice harboring a doxycycline inducible Oct4 allele and an Oct4-GFP reporter that has been demonstrated to be activated in cells upon acquisition of pluripotency. Oct4-GFP-positive blastocysts were collected at 3.5 dpc and transduced with lentiviruses encoding doxycycline inducible Sox2, Klf4 and cMyc transgenes (4FTSC). 4FTSC lines were passaged 10 times to establish a population of constantly growing, self-renewing TSCs in the presence of FGF4 and fibroblast conditioned media. To induce lineage conversion, 4FTSCs were cultured under ESC/Lif conditions and doxycycline. After 28 days, several colonies displaying ESC-characteristic dome-shaped colony morphology and bright Oct4-GFP fluorescence could be detected. The 4FTSC-derived colonies were isolated mechanically, dissociated by trypsinization, and plated onto MEFs in ESC medium without doxycycline demonstrating the independence of exogenous factors. They will be called TSC-iPSCs (Trophoblast stem cell derived induced pluripotent stem cells). To examine if the extraembryonic lineage-specific mRNA profile was overcome, the gene-expression profiles of TSC-iPSCs and their parental 4FTSCs were analyzed by microarray analyses and compared to control ESCs.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25872", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25872/samples/"}