{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM634249": {"CELL SURFACE MARKER": "EGFP-positive"}}, {"GSM634249": {"CELL SURFACE MARKER": "EGFP-positive"}}, {"GSM634249": {"CELL SURFACE MARKER": "EGFP-positive"}}, {"GSM634252": {"CELL SURFACE MARKER": "EGFP-negative"}}, {"GSM634252": {"CELL SURFACE MARKER": "EGFP-negative"}}, {"GSM634252": {"CELL SURFACE MARKER": "EGFP-negative"}}], "id": 6290, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25778", "summary_wrapped": "Pulmonary alveoli contain two distinct populations of epithelial cells.  Type II cells produce pulmonary surfactant lipids and...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cell", "gas", "influenza", "line", "liquid", "protein", "pulmonary surfactant", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "genome-profiling-of-mouse-pulmonary-epithelial-typ", "geo_id_plat": "E-GEOD-25778_A-AFFY-45", "name": "Genome profiling of mouse pulmonary epithelial type II cells", "created": "Nov.11, 2014", "summary": "Pulmonary alveoli contain two distinct populations of epithelial cells.  Type II cells produce pulmonary surfactant lipids and surfactant-associated proteins (SP) required for maintaining alveolar surface tension at the air-liquid interface and host defense against respiratory pathogens.  Type II cells are also progenitors for epithelial type I cells, a terminally differentiated elongated cell that covers microvascular endothelial cells and participates in gas exchange.   Despite some indirect evidence, it is unknown whether subpopulations of type II cells exist.   We created a line of transgenic mice expressing enhanced green fluorescent protein (EGFP) under control of the human SP-C promoter.  Expression of EGFP may define a subpopulation of type II cells because it is 1) expressed in approximately 10% of type II cells, 2) appears much later in embryonic development than SP-C, and 3) selectively proliferates in mice infected with influenza A virus.  To determine whether EGFP defines a unique subpopulation of type II cells, RNA was isolated from EGFP-positive and negative type II cells and hybridized to affymetrix arrays.  Of the genes detected in EGFP-positive cells, most were equally detected in EGFP-negative cells.  However, approximately 350 genes were selectively elevated \u22655-fold in EGFP-positive cells and 1500 genes selectively expressed by EGFP-negative cells.  These findings suggest EGFP defines a subpopulation of type II epithelial cells in this line of transgenic mice. Type II cells were harvested from approximately 10 young adult (8-week) mice and sorted into EGFP-positive (sample EGFP-1) and EGFP-negative (sample nonEGFP-1) populations using a B-D FACSVantage SE cell sorter.  RNA was prepared from approximately two million cells and hybridized to Affymetrix M430 2.0 array as a one by one comparison.  The isolation and gene expression analysis of EGFP-positive and EGFP-negative type II cells was repeated a second (using EGFP-2 and nonEGFP-2 samples) and third (using EGFP-3 and nonEGFP-3 samples) time.", "geo_gse_id": "E-GEOD-25778", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25778/samples/"}