ArrayExpress Uploader0humanmalepassage 50HPAM1 epithelial acinar cell lineuntreatedmalepassage 50HPAM1 epithelial acinar cell lineuntreatedmalepassage 50HPAM1 epithelial acinar cell lineuntreatedmalepassage 50HPAM1 epithelial acinar cell line5-Aza dC, TSAmalepassage 50HPAM1 epithelial acinar cell line5-Aza dC, TSAmalepassage 50HPAM1 epithelial acinar cell line5-Aza dC, TSAfemalepassage 40HPAF1 epithelial acinar cell lineuntreatedfemalepassage 40HPAF1 epithelial acinar cell lineuntreatedfemalepassage 40HPAF1 epithelial acinar cell lineuntreatedfemalepassage 40HPAF1 epithelial acinar cell line5-Aza dC, TSAfemalepassage 40HPAF1 epithelial acinar cell line5-Aza dC, TSAfemalepassage 40HPAF1 epithelial acinar cell line5-Aza dC, TSA2269arrayexpress_sid4Salivary gland adenoid cystic carcinoma (ACC) is a rare malignancy amongst head and neck tumors that is poorly understood on a molecular...E-GEOD-25746/profile/8773/arrayexpressuploader412carcinomacelldiseaseglandheadneckproteinsalivary glandsalivary gland adenoid cystic carcinomaDec.12, 2014Falseintegrated-genome-wide-screening-for-hypomethylateE-GEOD-25746_A-AFFY-44Integrated, genome-wide screening for hypomethylated oncogenes in salivary gland adenoid cystic carcinomaJul.11, 2014Salivary gland adenoid cystic carcinoma (ACC) is a rare malignancy amongst head and neck tumors that is poorly understood on a molecular level. We sought to perform a comprehensive approach for novel oncogene candidate screening under the control of promoter methylation in order to learn more about the molecular basis of this unusual disease. We performed global demethylation of normal salivary gland cell strains using 5-aza-deoxycytidine (5-Aza dC) and trichostatin (TSA). Expression arrays were performed, and the profiles of treated and untreated cells compared. We then used expression microarray analysis of primary ACC and normal salivary gland samples to generate ACC-specific expression profiling. Next, we integrated the two profiles to identify a subset of genes for further validation of decreased methylation in the promoter region in ACC vs normals. Finally, only genes that showed decreased methylation in ACC compared to normal were further validated for mRNA, protein, and promoter methylation levels in a larger ACC cohort.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25746http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25746/samples/