8ArrayExpress Uploader0mouseWTWTWTWTWTNFkBp65-/-NFkBp65-/-NFkBp65-/-NFkBp65-/-NFkBp65-/-8519arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25469Toxoplasma strains have been shown to modulate host cell transcription. We have found a type II Toxoplasma gene, GRA15, which activates...21199955/profile/8773/arrayexpressuploader110cellgenomeDec.12, 2014Falseexpression-data-from-wt-or-p65-mouse-embryonic-fibE-GEOD-25469_A-AFFY-36Expression data from WT or p65-/- mouse embryonic fibroblasts (MEFs) infected with Toxoplasma gondii.Nov.24, 2014Toxoplasma strains have been shown to modulate host cell transcription. We have found a type II Toxoplasma gene, GRA15, which activates the nuclear translocation of the NF-kappaB p65 transcription factor. We used microarrays to determine how GRA15II modulates host cell transcription, and whether this transcription is dependent on the p65 transcription factor. WT or p65-/- MEFs were infected with type I (RH), type I GRA15II (RH GRA15II), type II (Pru), or type II GRA15KO (Pru GRA15KO) parasites for 18-24 hours. Total RNA was isolated and hybridized to Affymetrix GeneChip Human Genome U133A 2.0 arrays.E-GEOD-25469http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25469/samples/