3ArrayExpress Uploader0human9652arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25468Toxoplasma strains have been shown to modulate host cell transcription. We have found a type II Toxoplasma gene, GRA15, which activates...21199955/profile/8773/arrayexpressuploader03cellgenomeJan.17, 2015Falseexpression-data-from-human-foreskin-fibroblasts-hfE-GEOD-25468_A-AFFY-33Expression data from Human foreskin fibroblasts (HFFs) infected with Toxoplasma gondii.Jan.17, 2015Toxoplasma strains have been shown to modulate host cell transcription. We have found a type II Toxoplasma gene, GRA15, which activates the nuclear translocation of the NF-kappaB p65 transcription factor. We used microarrays to determine how GRA15II modulates host cell transcription. HFFs were infected with type I (RH), type I GRA15II (RH GRA15II), type II (Pru), type II GRA15KO (Pru GRA15KO), type III (cep), or type III GRA15II (cep GRA15II) parasites for 18-24 hours. Some samples were also stimulated with TNF-alpha. Total RNA was isolated and hybridized to Affymetrix GeneChip Human Genome U133A 2.0 arrays.E-GEOD-25468http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25468/samples/