ArrayExpress Uploaderarrayexpress_sidmouse6 hoursPBS6 hours20% (v/v) naomeulsion (W805EC)24 hoursPBS24 hours20% (v/v) naomeulsion (W805EC)626906Antigen uptake, processing, trafficking and presentation in nasal mucosal tissues are regulated by complex intra- and inter-cellular...E-GEOD-25438/profile/8773/arrayexpressuploader24liquidmucosanasal epitheliumDec.12, 2014Falsegene-expression-data-in-murine-nasal-mucosa-followE-GEOD-25438_A-AFFY-45Gene expression data in murine nasal mucosa following nanoemulsion adjuvant exposureNov.11, 2014Antigen uptake, processing, trafficking and presentation in nasal mucosal tissues are regulated by complex intra- and inter-cellular signalling events. Typical vaccine adjuvants lead to the transcription of pro-inflammatory cytokines and chemokines which relate to immune induction. We used microarrays to detail the global expression of genes in murine nasal mucosa underlying immune induction with a non-inflammatory nanoemulsion nasal adjuvant. 8 week old female CD-1 mice were nasally treated with 5 microliters/nare of either 20% (v/v) naomeulsion (W805EC) or PBS. Nasal epithelium was harvested immediately post-mortem at either 6 (6 hr) or 24 hours (24 hr) following treatment. The tissue was placed in OTC and frozen by immersion in liquid nitrogen. Total RNA was extracted per sample using RNA easy (Qiagen).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25438http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25438/samples/