{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM624380": {"TIME POINT": "6 hours", "AGENT": "PBS"}}, {"GSM62438": {"TIME POINT": "6 hours", "AGENT": "20% (v/v) naomeulsion (W805EC)"}}, {"GSM624382": {"TIME POINT": "24 hours", "AGENT": "PBS"}}, {"GSM624383": {"TIME POINT": "24 hours", "AGENT": "20% (v/v) naomeulsion (W805EC)"}}], "id": 6269, "pop_total": 0, "platform": 6, "summary_wrapped": "Antigen uptake, processing, trafficking and presentation in nasal mucosal tissues are regulated by complex intra- and inter-cellular...", "geo_gse_id": "E-GEOD-25438", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["liquid", "mucosa", "nasal epithelium"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-data-in-murine-nasal-mucosa-follow", "geo_id_plat": "E-GEOD-25438_A-AFFY-45", "name": "Gene expression data in murine nasal mucosa following nanoemulsion adjuvant exposure", "created": "Nov.11, 2014", "summary": "Antigen uptake, processing, trafficking and presentation in nasal mucosal tissues are regulated by complex intra- and inter-cellular signalling events.  Typical vaccine adjuvants lead to the transcription of pro-inflammatory cytokines and chemokines which relate to immune induction. We used microarrays to detail the global expression of genes in murine nasal mucosa underlying immune induction with a non-inflammatory nanoemulsion nasal adjuvant. 8 week old female CD-1 mice were nasally treated with 5 microliters/nare of either  20% (v/v) naomeulsion (W805EC) or PBS.  Nasal epithelium was harvested immediately post-mortem at either 6 (6 hr) or 24 hours (24 hr) following treatment.  The tissue was placed in OTC and frozen by immersion in liquid nitrogen.  Total RNA was extracted per sample using RNA easy (Qiagen).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25438", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25438/samples/"}