ArrayExpress Uploaderarrayexpress_sidmouse24h19scrambled24h19scrambled24h19LSD124h19LSD148h21scrambled48h21scrambled48h21scrambled48h21LSD148h21LSD148h21LSD1626806Obesity is often associated with a low-grade systemic inflammation state that contributes to the development of insulin resistance and...E-GEOD-25423/profile/8773/arrayexpressuploader310adipocyteadipose tissuehistoneinsulinmacrophageobesityDec.12, 2014Falselsd1-knockdown-in-3t3-l1-preadipocytesE-GEOD-25423_A-AFFY-45LSD1 knockdown in 3T3-L1 preadipocytesNov.11, 2014Obesity is often associated with a low-grade systemic inflammation state that contributes to the development of insulin resistance and atherosclerotic complications. This is usually coupled with increased macrophage infiltration in the adipose tissue and a defect in adipocyte differentiation that results in accumulation of hypertrophic fat cells characterized by a deregulated pattern of adipokine expression. Here we show that knockdown of histone demethylase lsd1 in 3T3-L1 preadipocytes results in defective adipogenesis and derepression of an inflammatory program in these cells. The dataset consists of four sample groups: [1] 3T3-L1 preadipocytes (passage 19) transfected with a control scrambled siRNA at 24h after transfection (siC.24h), [2] 3T3-L1 preadipocytes (p.19) transfected with a siRNA directed against LSD1 at 24h after transfection (siLsd1.24h), [3] 3T3-L1 preadipocytes (p.21) transfected with a control scrambled siRNA at 48h after transfection (siC.48h), and [4] 3T3-L1 preadipocytes (p.21) transfected with a siRNA directed against LSD1 at 48h after transfection (siLsd1.48h). The 24h sample groups (siC.24h and siLsd1.24h) consist of two biological replicate samples; the 48h sample groups (siC.48h and siLsd1.48h) consist of three biological replicate samples. Each sample was hybridized to a separate array, for a total of ten arrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25423http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25423/samples/