{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM62412": {"TRANSFECTION TIME": "24h", "CELL PASSAGE NUMBER": 19, "SIRNA": "scrambled"}}, {"GSM62412": {"TRANSFECTION TIME": "24h", "CELL PASSAGE NUMBER": 19, "SIRNA": "scrambled"}}, {"GSM624123": {"TRANSFECTION TIME": "24h", "CELL PASSAGE NUMBER": 19, "SIRNA": "LSD1"}}, {"GSM624123": {"TRANSFECTION TIME": "24h", "CELL PASSAGE NUMBER": 19, "SIRNA": "LSD1"}}, {"GSM624125": {"TRANSFECTION TIME": "48h", "CELL PASSAGE NUMBER": 21, "SIRNA": "scrambled"}}, {"GSM624125": {"TRANSFECTION TIME": "48h", "CELL PASSAGE NUMBER": 21, "SIRNA": "scrambled"}}, {"GSM624125": {"TRANSFECTION TIME": "48h", "CELL PASSAGE NUMBER": 21, "SIRNA": "scrambled"}}, {"GSM624128": {"TRANSFECTION TIME": "48h", "CELL PASSAGE NUMBER": 21, "SIRNA": "LSD1"}}, {"GSM624128": {"TRANSFECTION TIME": "48h", "CELL PASSAGE NUMBER": 21, "SIRNA": "LSD1"}}, {"GSM624128": {"TRANSFECTION TIME": "48h", "CELL PASSAGE NUMBER": 21, "SIRNA": "LSD1"}}], "id": 6268, "pop_total": 0, "platform": 6, "summary_wrapped": "Obesity is often associated with a low-grade systemic inflammation state that contributes to the development of insulin resistance and...", "geo_gse_id": "E-GEOD-25423", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 10, "tags": ["adipocyte", "adipose tissue", "histone", "insulin", "macrophage", "obesity"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "lsd1-knockdown-in-3t3-l1-preadipocytes", "geo_id_plat": "E-GEOD-25423_A-AFFY-45", "name": "LSD1 knockdown in 3T3-L1 preadipocytes", "created": "Nov.11, 2014", "summary": "Obesity is often associated with a low-grade systemic inflammation state that contributes to the development of insulin resistance and atherosclerotic complications. This is usually coupled with increased macrophage infiltration in the adipose tissue and a defect in adipocyte differentiation that results in accumulation of hypertrophic fat cells characterized by a deregulated pattern of adipokine expression. Here we show that knockdown of histone demethylase lsd1 in 3T3-L1 preadipocytes results in defective adipogenesis and derepression of an inflammatory program in these cells. The dataset consists of four sample groups: [1] 3T3-L1 preadipocytes (passage 19) transfected with a control scrambled siRNA at 24h after transfection (siC.24h), [2] 3T3-L1 preadipocytes (p.19) transfected with a siRNA directed against LSD1 at 24h after transfection (siLsd1.24h), [3] 3T3-L1 preadipocytes (p.21) transfected with a control scrambled siRNA at 48h after transfection (siC.48h), and [4] 3T3-L1 preadipocytes (p.21) transfected with a siRNA directed against LSD1 at 48h after transfection (siLsd1.48h). The 24h sample groups (siC.24h and siLsd1.24h) consist of two biological replicate samples; the 48h sample groups (siC.48h and siLsd1.48h) consist of three biological replicate samples. Each sample was hybridized to a separate array, for a total of ten arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25423", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25423/samples/"}