{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM621280 1": {}}, {"GSM621281 1": {}}, {"GSM621282 1": {}}, {"GSM621283 1": {}}, {"GSM621284 1": {}}, {"GSM621285 1": {}}, {"GSM621286 1": {}}, {"GSM621287 1": {}}, {"GSM621288 1": {}}, {"GSM621289 1": {}}], "id": 6263, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25252", "summary_wrapped": "Analysis of Foxp3(+)epigenetics(-) T cells, Foxp3(-)epigenetics(+) T cells, and Foxp3(+)epigenetics(+) T cells. Results indicate...", "pubmed_id": 23123060, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 10, "tags": ["cell", "genome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "comparison-of-expression-profiles-of-foxp3epigenet", "geo_id_plat": "E-GEOD-25252_A-AFFY-45", "name": "Comparison of expression profiles of Foxp3(+)epigenetics(-) T cells, Foxp3(-)epigenetics(+) T cells, and Foxp3(+)epigenetics(+) T cells", "created": "Nov.11, 2014", "summary": "Analysis of Foxp3(+)epigenetics(-) T cells, Foxp3(-)epigenetics(+) T cells, and Foxp3(+)epigenetics(+) T cells. Results indicate regulatory T cell (Treg) ontogenesis requires two independent processes, expression of the transcription factor Foxp3 and establishment of Treg epigenetic programs induced by T cell receptor (TCR) stimulation. GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis (Affymetrix, mouse genome 430 2.0 array). To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector. Two replicates each.", "geo_gse_id": "E-GEOD-25252", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25252/samples/"}