6ArrayExpress Uploader0mousecontrol1controlPPARg +/-LysCrecontrol1rosiglitazonePPARg +/-LysCreIL-41controlPPARg +/-LysCreIL-41rosiglitazonePPARg +/-LysCrecontrol2controlPPARg +/-LysCrecontrol2rosiglitazonePPARg +/-LysCreIL-42controlPPARg +/-LysCreIL-42rosiglitazonePPARg +/-LysCrecontrol3controlPPARg +/-LysCrecontrol3rosiglitazonePPARg +/-LysCreIL-43controlPPARg +/-LysCreIL-43rosiglitazonePPARg +/-LysCrecontrol4controlPPARg fl/-LysCrecontrol4rosiglitazonePPARg fl/-LysCreIL-44controlPPARg fl/-LysCreIL-44rosiglitazonePPARg fl/-LysCrecontrol5controlPPARg fl/-LysCrecontrol5rosiglitazonePPARg fl/-LysCreIL-45controlPPARg fl/-LysCreIL-45rosiglitazonePPARg fl/-LysCrecontrol6controlPPARg fl/-LysCrecontrol6rosiglitazonePPARg fl/-LysCreIL-46controlPPARg fl/-LysCreIL-46rosiglitazonePPARg fl/-LysCre6258arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25123Conditional macrophage-specific PPARg knockout mice were generated on C57Bl/6 background by breeding PPARg fl/- (one allele is floxed,...21093321/profile/8773/arrayexpressuploader424bonebone marrowgenomemacrophageDec.12, 2014Falsepparg-and-il-4-induced-gene-expression-data-from-pE-GEOD-25123_A-AFFY-45PPARg and IL-4-induced gene expression data from PPARg +/- LysCre and PPARg fl/- LysCre mouse bone marrow-derived macrophagesNov.11, 2014Conditional macrophage-specific PPARg knockout mice were generated on C57Bl/6 background by breeding PPARg fl/- (one allele is floxed, the other is null) and lysozyme Cre transgenic mice. PPARg and IL-4 signaling was analyzed on bone marrow-derived macrophages. Bone marrow of 3 mice per group was isolated and differentiated to macrophages with M-CSF (20 ng/ml). 20 ng/ml IL-4 was used to induce alternative macrophage activation and 1 uM Rosiglitazone (RSG) was used to activate PPARg. From each mouse 4 samples were generated: 1. M-CSF, 2. M-CSF+RSG, 3. IL-4 and 4. IL-4+RSG. All compounds were added throughout the whole differentiation process, and fresh media was added every other day. Control cells were treated with vehicle (DMSO:ethanol). After 10 days, RNA was isolated and gene expression profiles were analyzed using Mouse Genome 430 2.0 microarrays from Affymetrix. 3 PPARg +/- LysCre and 3 PPARg fl/- LysCre mice were used to isolate bone marrow and from each macrophages were differentiated with or without IL-4 and simultaneously treated with vehicle or RSG. Altogether we analyzed 24 samples with 3 biological replicates as below.E-GEOD-25123http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25123/samples/