6ArrayExpress Uploader0mousecontrol1controlWild-typecontrol1rosiglitazoneWild-typeIL-41controlWild-typeIL-41rosiglitazoneWild-typecontrol2controlWild-typecontrol2rosiglitazoneWild-typeIL-42controlWild-typeIL-42rosiglitazoneWild-typecontrol3controlWild-typecontrol3rosiglitazoneWild-typeIL-43controlWild-typeIL-43rosiglitazoneWild-typecontrol4controlSTAT6 KOcontrol4rosiglitazoneSTAT6 KOIL-44controlSTAT6 KOIL-44rosiglitazoneSTAT6 KOcontrol5controlSTAT6 KOcontrol5rosiglitazoneSTAT6 KOIL-45controlSTAT6 KOIL-45rosiglitazoneSTAT6 KOcontrol6controlSTAT6 KOcontrol6rosiglitazoneSTAT6 KOIL-46controlSTAT6 KOIL-46rosiglitazoneSTAT6 KO6256arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25088C57Bl/6 wild-type and STAT6 KO mice were used to study PPARg and IL-4 signaling. Bone marrow of 3 mice per group was isolated and...21093321/profile/8773/arrayexpressuploader424bonebone marrowgenomemacrophageDec.12, 2014Falsepparg-and-il-4-induced-gene-expression-data-from-wE-GEOD-25088_A-AFFY-45PPARg and IL-4-induced gene expression data from wild-type and STAT6 knockout mouse bone marrow-derived macrophagesNov.11, 2014C57Bl/6 wild-type and STAT6 KO mice were used to study PPARg and IL-4 signaling. Bone marrow of 3 mice per group was isolated and differentiated to macrophages with M-CSF (20 ng/ml). 20 ng/ml IL-4 was used to induce alternative macrophage activation and 1 uM Rosiglitazone (RSG) was used to activate PPARg. From each mouse 4 samples were generated: 1. M-CSF, 2. M-CSF+RSG, 3. IL-4 and 4. IL-4+RSG. All compounds were added throughout the whole differentiation process, and frech media was added every other day. Control cells were treated with vehicle (DMSO:ethanol). After 10 days, RNA was isolated and gene expression profiles were analyzed using Mouse Genome 430 2.0 microarrays from Affymetrix. 3 C57Bl/6 wild-type and 3 STAT6 KO mice were used to isolate bone marrow and from each macrophages were differentiated with or without IL-4 and simultaneously treated with vehicle or RSG. Altogether we analyzed 24 samples with 3 biological replicates as below.E-GEOD-25088http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-25088/samples/