{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM611008": {"TREATMENT": "control"}}, {"GSM611008": {"TREATMENT": "control"}}, {"GSM611008": {"TREATMENT": "control"}}, {"GSM611008": {"TREATMENT": "control"}}, {"GSM611012": {"TREATMENT": "anti-miR-182"}}, {"GSM611012": {"TREATMENT": "anti-miR-182"}}, {"GSM611012": {"TREATMENT": "anti-miR-182"}}, {"GSM611012": {"TREATMENT": "anti-miR-182"}}], "id": 3840, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "To identify genes differentially modulated by anti-miR-182 treatment in a liver melanoma metastasis mouse model. Targeting oncogenic...", "geo_gse_id": "E-GEOD-24824", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["cancer", "liver", "liver metastasis", "melanoma", "metastatic melanoma", "solid", "spleen"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-24824_A-AFFY-44", "slug": "identification-of-genes-and-molecular-pathways-ass", "geo_gds_id": "", "name": "Identification of genes and molecular pathways associated with anti-miR-182 treatment.", "created": "Sep.15, 2014", "summary": "To identify genes differentially modulated by anti-miR-182 treatment in a liver melanoma metastasis mouse model. Targeting oncogenic microRNAs is emerging as a promising strategy for cancer therapy. Here we provide proof-of-principle for the safety and efficacy of miRNA targeting against metastatic tumors. We tested the effect of targeting miR-182, a pro-metastatic miRNA frequently overexpressed in melanoma, whose silencing represses invasion and induces apoptosis in vitro. In particular, we assessed the effect of anti-miR-182 oligonucleotides synthesized with 2\u2019 sugar modifications and a phosphorothioate backbone in a mouse model of melanoma liver metastasis. Luciferase imaging showed that mice treated with anti-miR-182 had an appreciably lower burden of liver metastases compared to the control. We confirmed that miR-182 levels were effectively downregulated in the anti-miR treated tumors relative to the scrambled treated tumor both in the liver and in the spleen. This downregulation was accompanied by an upregulation of miR-182 direct targets. Transcriptome analysis of mouse tissues treated with anti-miR-182 or scramble oligonucleotides revealed an enrichment for genes controlling survival, adhesion and migration modulated in response to anti-miR-182 treatment. These data indicate that in vivo administration of anti-miRs allows for efficient miRNA targeting and concomitant upregulation of target levels. Our results suggest that the use of anti-miR-182 is a promising therapeutic strategy for metastatic melanoma and provide solid proof-of-principle for similar strategies against other metastatic tumors.  Keywords: Differentially expressed genes (mRNAs) in response to miRNA inhibition Quadruplicate (n=4) samples of anti-miR-182 treated human melanoma metastasis compared to quadruplicate control treated metastasis.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24824", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24824/samples/"}