{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM60920": {"CELL TYPE": "293T cells transfected with pCMV-Aire expression vector"}}, {"GSM609202": {"CELL TYPE": "293T cells transfected with pCMV empty expression vector"}}, {"GSM60920": {"CELL TYPE": "293T cells transfected with pCMV-Aire expression vector"}}, {"GSM60920": {"CELL TYPE": "293T cells transfected with pCMV-Aire expression vector"}}, {"GSM609202": {"CELL TYPE": "293T cells transfected with pCMV empty expression vector"}}], "id": 3830, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "To analyze the impact of Aire on gene expression profile in a model cell line, we used 293T cells and transfected them either with an...", "geo_gse_id": "E-GEOD-24733", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 5, "tags": ["cell", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-profile-of-293t-and-293t-aire-expr", "geo_id_plat": "E-GEOD-24733_A-AFFY-44", "name": "Gene Expression Profile of 293T and 293T-Aire-expressing cells", "created": "Sep.15, 2014", "summary": "To analyze the impact of Aire on gene expression profile in a model cell line, we used 293T cells and transfected them either with an Aire expression plasmid pCMV-Aire (where mAire is driven by CMV promoter) or with a control plasmid pCMV2B. Total RNA was extracted 48 hours post transfection, processed and used for gene expression profiling by Affymetrix. The data demonstrate that Aire has a very broad impact, effecting (upregulating and downregulating) hundreds of differents genes, however these genes differ dramatically from its targets in medullary epithelial cells.  Keywords: transfection 293T cells were seeded on 10cm plates and were grown in DMEM containing 10% FBS under standard TC conditions. Next day, the cells were transfected either by pCMV-Aire (6ug/plate) or empty pCMV (6ug/plate) using Mirus reagent, according to manufacturer's instructions. Total RNA was extracted 48 hrs post transfection by Trizol protocol according to the manufacturer's instructions. 3 biological replicates were used for Aire transfection and 2 replicates for transfection with a control plasmid.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24733", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24733/samples/"}