ArrayExpress Uploaderarrayexpress_sid6242C57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM1_6_5nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM1_6_5nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM1_6_5nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM1_8_3nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM1_8_3nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM1_8_3nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM2_3_3nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM2_3_3nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM2_3_3nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM2_4_3nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM2_4_3nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentiPS cell line ntiPSM2_4_3nuclear transfer ES cells from induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM1_6induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM1_6induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM1_6induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM1_8induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM1_8induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM1_8induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM2_3induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM2_3induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM2_3induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM2_4induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM2_4induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaeiPS cell line iPSM2_4induced pluripotent stem cellsC57BL/6×129sv/Jae F1 ×129sv/JaentES cells cell line NTM1_7nuclear transfer ES cellsC57BL/6×129sv/Jae F1 ×129sv/JaentES cells cell line NTM1_7nuclear transfer ES cellsC57BL/6×129sv/Jae F1 ×129sv/JaentES cells cell line NTM1_7nuclear transfer ES cellsC57BL/6×129sv/Jae F1 ×129sv/JaentES cells cell line NTM2_4nuclear transfer ES cellsC57BL/6×129sv/Jae F1 ×129sv/JaentES cells cell line NTM2_4nuclear transfer ES cellsC57BL/6×129sv/Jae F1 ×129sv/JaentES cells cell line NTM2_4nuclear transfer ES cells129sv/JaeES cells cell line E14embryonic stem cells129sv/JaeES cells cell line E14embryonic stem cells129sv/JaeES cells cell line E14embryonic stem cells06We generated three kinds of genetically identical mouse reprogrammed cells: induced pluripotent stem cells (iPSCs), nuclear transfer...E-GEOD-24705/profile/8773/arrayexpressuploader333celloocytepluripotent stem cellstem cellDec.12, 2014Falsemrna-expression-data-from-ipscs-ntescs-and-ipsc-ntE-GEOD-24705_A-AFFY-45mRNA expression data from iPSCs, ntESCs and iPSC-nt-ESCsNov.11, 2014We generated three kinds of genetically identical mouse reprogrammed cells: induced pluripotent stem cells (iPSCs), nuclear transfer embryonic stem cells (ntESCs) and iPSC-nt-ESCs that are established after successively reprogramming of iPSCs by nuclear transfer (NT). NtESCs show better developmental potential than iPSCs, whereas iPSC-nt-ESCs display worse developmental potential than iPSCs. We used microarrays to distinguish the gene expression differences among three pluriptoent stem cells and identified that imprinted genes had a similar expression pattern in iPSCs and iPSC-nt-ESCs. We sought to obtain genetic identical pluripotent stem cell lines in order to minimize genetic variations among different reprogrammed cells. To that end, we established a genetically homogenous secondary reprogramming system, in which mouse embryonic fibroblasts (MEFs) carrying doxycycline (dox)-inducible lentiviruses expressing Oct4, Sox2, Klf4 and c-Myc were isolated and used as donors for different reprogramming experiments. We generated iPSCs after plating MEFs in the presence of dox in ES culture conditions, and then derived ntESCs after transplantation of the nucleus from the same MEFs into enucleated oocyte. Furthermore, we successively reprogrammed iPSCs by means of NT and established a set of nt-iPSC lines. Pluripotent stem cells generated from different reprogramming strategies were for RNA extraction and hybridization on Affymetrix microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24705mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24705/samples/