{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 6242, "factors": [{"GSM638073": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM1_6_5", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638073": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM1_6_5", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638073": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM1_6_5", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638076": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM1_8_3", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638076": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM1_8_3", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638076": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM1_8_3", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638079": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM2_3_3", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638079": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM2_3_3", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638079": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM2_3_3", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638082": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM2_4_3", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638082": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM2_4_3", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638082": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntiPS cell line ntiPSM2_4_3", "CELL TYPE": "nuclear transfer ES cells from induced pluripotent stem cells"}}, {"GSM638085": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM1_6", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM638085": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM1_6", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM638085": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM1_6", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM638088": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM1_8", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM638088": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM1_8", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM638088": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM1_8", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM63809": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM2_3", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM63809": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM2_3", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM63809": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM2_3", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM638094": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM2_4", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM638094": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM2_4", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM638094": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "iPS cell line iPSM2_4", "CELL TYPE": "induced pluripotent stem cells"}}, {"GSM638097": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntES cells cell line NTM1_7", "CELL TYPE": "nuclear transfer ES cells"}}, {"GSM638097": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntES cells cell line NTM1_7", "CELL TYPE": "nuclear transfer ES cells"}}, {"GSM638097": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntES cells cell line NTM1_7", "CELL TYPE": "nuclear transfer ES cells"}}, {"GSM638100": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntES cells cell line NTM2_4", "CELL TYPE": "nuclear transfer ES cells"}}, {"GSM638100": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntES cells cell line NTM2_4", "CELL TYPE": "nuclear transfer ES cells"}}, {"GSM638100": {"STRAIN": "C57BL/6\u00d7129sv/Jae F1 \u00d7129sv/Jae", "CELL LINE": "ntES cells cell line NTM2_4", "CELL TYPE": "nuclear transfer ES cells"}}, {"GSM638103": {"STRAIN": "129sv/Jae", "CELL LINE": "ES cells cell line E14", "CELL TYPE": "embryonic stem cells"}}, {"GSM638103": {"STRAIN": "129sv/Jae", "CELL LINE": "ES cells cell line E14", "CELL TYPE": "embryonic stem cells"}}, {"GSM638103": {"STRAIN": "129sv/Jae", "CELL LINE": "ES cells cell line E14", "CELL TYPE": "embryonic stem cells"}}], "pop_total": 0, "platform": 6, "summary_wrapped": "We generated three kinds of genetically identical mouse reprogrammed cells: induced pluripotent stem cells (iPSCs), nuclear transfer...", "geo_gse_id": "E-GEOD-24705", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 33, "tags": ["cell", "oocyte", "pluripotent stem cell", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "mrna-expression-data-from-ipscs-ntescs-and-ipsc-nt", "geo_id_plat": "E-GEOD-24705_A-AFFY-45", "name": "mRNA expression data from iPSCs, ntESCs and iPSC-nt-ESCs", "created": "Nov.11, 2014", "summary": "We generated three kinds of genetically identical mouse reprogrammed cells: induced pluripotent stem cells (iPSCs), nuclear transfer embryonic stem cells (ntESCs) and iPSC-nt-ESCs that are established after successively reprogramming of iPSCs by nuclear transfer (NT). NtESCs show better developmental potential than iPSCs, whereas iPSC-nt-ESCs display worse developmental potential than iPSCs. We used microarrays to distinguish the gene expression differences among three pluriptoent stem cells and identified that imprinted genes had a similar expression pattern in iPSCs and iPSC-nt-ESCs. We sought to obtain genetic identical pluripotent stem cell lines in order to minimize genetic variations among different reprogrammed cells. To that end, we established a genetically homogenous secondary reprogramming system, in which mouse embryonic fibroblasts (MEFs) carrying doxycycline (dox)-inducible lentiviruses expressing Oct4, Sox2, Klf4 and c-Myc were isolated and used as donors for different reprogramming experiments. We generated iPSCs after plating MEFs in the presence of dox in ES culture conditions, and then derived ntESCs after transplantation of the nucleus from the same MEFs into enucleated oocyte. Furthermore, we successively reprogrammed iPSCs by means of NT and established a set of nt-iPSC lines.  Pluripotent stem cells generated from different reprogramming strategies were for RNA extraction and hybridization on Affymetrix microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24705", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24705/samples/"}