ArrayExpress Uploaderarrayexpress_sidhumanCD25+ T celldonor 2no IL4CD25+day 10CD25+ T celldonor 1no IL4CD25+day 10CD25- T celldonor 3no IL4CD25-day 10CD25- T celldonor 2no IL4CD25-day 10CD25- T celldonor 1no IL4CD25-day 10CD25+ T celldonor 3IL4CD25+day 10CD25+ T celldonor 2IL4CD25+day 10CD25+ T celldonor 1IL4CD25+day 10CD25- T celldonor 3IL4CD25-day 10CD25- T celldonor 2IL4CD25-day 10CD25- T celldonor 1IL4CD25-day 10CD25+ T celldonor 3no IL4CD25+day 7CD25+ T celldonor 2no IL4CD25+day 7CD25+ T celldonor 1no IL4CD25+day 7CD25- T celldonor 3no IL4CD25-day 7CD25- T celldonor 2no IL4CD25-day 7CD25- T celldonor 1no IL4CD25-day 7CD25+ T celldonor 3IL4CD25+day 7CD25+ T celldonor 2IL4CD25+day 7CD25+ T celldonor 1IL4CD25+day 7CD25- T celldonor 3IL4CD25-day 7CD25- T celldonor 2IL4CD25-day 7CD25- T celldonor 1IL4CD25-day 7CD25+ T celldonor 3no IL4CD25+day 5CD25+ T celldonor 2no IL4CD25+day 5CD25+ T celldonor 1no IL4CD25+day 5CD25- T celldonor 3no IL4CD25-day 5CD25- T celldonor 2no IL4CD25-day 5CD25- T celldonor 1no IL4CD25-day 5CD25+ T celldonor 3IL4CD25+day 5CD25+ T celldonor 2IL4CD25+day 5CD25+ T celldonor 1IL4CD25+day 5CD25- T celldonor 3IL4CD25-day 5CD25- T celldonor 2IL4CD25-day 5CD25- T celldonor 1IL4CD25-day 5CD25+ T celldonor 3no IL4CD25+day 3CD25+ T celldonor 2no IL4CD25+day 3CD25+ T celldonor 1no IL4CD25+day 3CD25- T celldonor 3no IL4CD25-day 3CD25- T celldonor 2no IL4CD25-day 3CD25- T celldonor 1no IL4CD25-day 3CD25+ T celldonor 3IL4CD25+day 3CD25+ T celldonor 2IL4CD25+day 3CD25+ T celldonor 1IL4CD25+day 3CD25- T celldonor 3IL4CD25-day 3CD25- T celldonor 2IL4CD25-day 3CD25- T celldonor 1IL4CD25-day 3naive T celldonor 3no IL4CD25-day 0naive T celldonor 2no IL4CD25-day 0naive T celldonor 1no IL4CD25-day 0132303CD25+ regulatory T cells develop in the thymus (nTregs), but may also be generated in the periphery upon stimulation of naive CD4 T cells...21347372E-GEOD-24634/profile/8773/arrayexpressuploader550interleukinperipheralthymusDec.12, 2014Falseexpression-data-from-developing-regulatory-t-cellsE-GEOD-24634_A-AFFY-33Expression data from developing regulatory T cellsJun.19, 2014CD25+ regulatory T cells develop in the thymus (nTregs), but may also be generated in the periphery upon stimulation of naive CD4 T cells under appropriate conditions (iTregs). The mechanisms that regulate the generation of peripheral iTregs are largely unknown. We used microarrays to gain insights into the molecular program of extrathymic Treg development Human iTregs were generated in vitro from CD4CD25- naive T cells in the presence of autologous feeder cells and interleukin (IL)-4. Total RNA was isolated at start and from purified CD25+ and CD25- T cells at days 3, 5, 7, and 10 of culture and used for DNA microarrays. CD25+ T cells generated in the absence of IL-4 constituted activated effector T cells (Teffs) lacking regulatory capacity and were therefore used to control for Treg-specific gene expression.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24634http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24634/samples/