{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM60606": {"TREATMENT": "4\u2032-iodo-3,3,5-tripropyl-4-methoxy"}}, {"GSM606062": {"TREATMENT": "control"}}], "id": 3829, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "In our continuing study of the desmosdumotin C (1) series, twelve new analogues, 21\u201332, mainly with A-ring modifications, were prepared...", "geo_gse_id": "E-GEOD-24584", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["cancer", "cell", "chromosome", "genome", "line", "lung", "lung cancer", "spindle"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "the-antitumor-mechanism-of-desmosdumotin-c-analog", "geo_id_plat": "E-GEOD-24584_A-AFFY-44", "name": "The antitumor mechanism of Desmosdumotin C Analog", "created": "Sep.15, 2014", "summary": "In our continuing study of the desmosdumotin C (1) series, twelve new analogues, 21\u201332, mainly with A-ring modifications, were prepared and evaluated for in vitro anticancer activity against several human tumor cell lines. Among them, the 4\u2032-iodo-3,3,5-tripropyl-4-methoxy analogue (31) showed significant cytotoxicity against multiple human tumor cell lines with ED50 values of 1.1\u20132.8 microM. Elongation of the C-3 and C-5 carbon chains reduced activity relative to propyl substituted analogues; however, activity was still better than that of natural 1. Among analogues with various ether groups on C-4, compounds with methyl (2) and propyl (26) ethers inhibited cell growth of multiple tumor cells lines, while 28 with an iso-butyl ether showed selective cytotoxicity against lung cancer A549 cells (ED50 1.7 microM). The gene expression profiles showed that 3 may modulate the spindle assembly checkpoint (SAC) and chromosome separation, and thus, arrest cells at the G2/M-phase. We used microarrays to elucidate the underlying antitumor mechanism induced by Desmosdumotin C Analog Analogue 3 showed potent cytotoxicity against the highly invasive non-small-cell lung cancer cell line CL1-5 with an ED50 value of 0.11 \u00b5M. To determine which genes were differentially expressed upon CL1-5 treatment with analogue 3, the genome-wide mRNA expression profiles of 3-treated cells and control cells were determined using Affymetrix human genome U133 plus 2.0 GeneChip according to the Manufacturer\u2019s protocols", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24584", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24584/samples/"}