Dataset: Expression data from BCL6-YFP-positive Tfh cells, BCL6-YFP-negative Tfh cells, non-Tfh cells, and naïve helper T cells.
We found that a number of Tfh cells downmodulated BCL6 protein after their development, and we sought to compare the gene expression...
We found that a number of Tfh cells downmodulated BCL6 protein after their development, and we sought to compare the gene expression between BCL6-hi Tfh cells and BCL6-low Tfh cells. CD4+ T cells were sorted from immunized and non-immunized mice for RNA extraction and hybridization on Affymetrix microarrays. Bcl6yfp/+ OT-II cells were transferred to congenic recipient mice, and immunized with NP-OVA in CFA subcutaneously. Seven or ten days after immunization, cells were collected from draining lymph nodes, and sorted on FACSAria by the expression of CXCR5, PD-1 and BCL6-YFP. Naive CD4+ T cells were CD4+ CD44lo CD62Lhi cells from unimmunized mice.
- Species:
- mouse
- Samples:
- 19
- Source:
- E-GEOD-24574
- PubMed:
- 21636294
- Updated:
- Dec.12, 2014
- Registered:
- Nov.11, 2014
| Sample | IMMUNIZATION STATUS | CELL TYPE | ORGANISM PART |
|---|---|---|---|
| GSM605702 | immunized mice (Day 7) | CXCR5hi PD-1hi YFP-BCL6+ cells | Lymph Nodes |
| GSM605702 | immunized mice (Day 7) | CXCR5hi PD-1hi YFP-BCL6+ cells | Lymph Nodes |
| GSM605702 | immunized mice (Day 7) | CXCR5hi PD-1hi YFP-BCL6+ cells | Lymph Nodes |
| GSM605705 | immunized mice (Day 10) | CXCR5hi PD-1hi YFP-BCL6+ cells | Lymph Nodes |
| GSM605706 | immunized mice (Day 10) | CXCR5hi PD-1hi YFP-BCL6- cells | Lymph Nodes |
| GSM605707 | immunized mice (Day 7) | CXCR5hi PD-1hi YFP-BCL6- cells | Lymph Nodes |
| GSM605707 | immunized mice (Day 7) | CXCR5hi PD-1hi YFP-BCL6- cells | Lymph Nodes |
| GSM605706 | immunized mice (Day 10) | CXCR5hi PD-1hi YFP-BCL6- cells | Lymph Nodes |
| GSM605710 | immunized mice (Day 7) | CXCR5lo PD-1lo cells | Lymph Nodes |
| GSM605710 | immunized mice (Day 7) | CXCR5lo PD-1lo cells | Lymph Nodes |
| GSM605710 | immunized mice (Day 7) | CXCR5lo PD-1lo cells | Lymph Nodes |
| GSM605713 | na�ve mouse | Naive CD4+ T cells | spleen |
| GSM687179 | immunized mice (Day 7) | Bcl6yfp-hetero CXCR5hi PD-1hi | Lymph Nodes |
| GSM687179 | immunized mice (Day 7) | Bcl6yfp-hetero CXCR5hi PD-1hi | Lymph Nodes |
| GSM687179 | immunized mice (Day 7) | Bcl6yfp-hetero CXCR5hi PD-1hi | Lymph Nodes |
| GSM687182 | immunized mice (Day 10) | Bcl6yfp-wt CXCR5hi PD-1hi cells | Lymph Nodes |
| GSM687182 | immunized mice (Day 10) | Bcl6yfp-wt CXCR5hi PD-1hi cells | Lymph Nodes |
| GSM687184 | immunized mice (Day 7) | Bcl6yfp-wt CXCR5hi PD-1hi cells | Lymph Nodes |
| GSM687185 | na�ve mouse | Na�ve CD4+ T cells | spleen |