<?xml version="1.0" encoding="ASCII"?>
<biogps><data><item key="platform">6</item><item key="owner">ArrayExpress Uploader</item><item key="pop_total">0</item><item key="species">mouse</item><item key="factors"><item><item key="GSM597445"><item key="PROTOCOL">control</item><item key="AGENT">untreated</item><item key="TIME">day 3 of differentiation</item></item></item><item><item key="GSM597446"><item key="PROTOCOL">inducible expression of miR-200c/miR-141</item><item key="AGENT">doxycycline</item><item key="TIME">day 3 of differentiation</item></item></item><item><item key="GSM597447"><item key="PROTOCOL">control</item><item key="AGENT">untreated</item><item key="TIME">day 4 of differentiation</item></item></item><item><item key="GSM597448"><item key="PROTOCOL">inducible expression of miR-200c/miR-141</item><item key="AGENT">doxycycline</item><item key="TIME">day 4 of differentiation</item></item></item><item><item key="GSM597449"><item key="PROTOCOL">control</item><item key="AGENT">untreated</item><item key="TIME">day 5 of differentiation</item></item></item><item><item key="GSM597450"><item key="PROTOCOL">inducible expression of miR-200c/miR-141</item><item key="AGENT">doxycycline</item><item key="TIME">day 5 of differentiation</item></item></item></item><item key="id">6221</item><item key="ownerprofile_id">arrayexpress_sid</item><item key="source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24289</item><item key="summary_wrapped">ES cells express the miR-200 family which becomes down-regulated during the course of differentiation in serum.  We generated an ES cell...</item><item key="owner_profile">/profile/8773/arrayexpressuploader</item><item key="factor_count">3</item><item key="sample_count">6</item><item key="tags"><item>cell</item><item>line</item><item>serum</item></item><item key="lastmodified">Dec.12, 2014</item><item key="is_default">False</item><item key="geo_gds_id"/><item key="slug">expression-data-from-differentiating-es-cells-expr</item><item key="geo_id_plat">E-GEOD-24289_A-AFFY-45</item><item key="name">Expression data from differentiating ES cells expressing miR-200c and miR-141</item><item key="created">Nov.11, 2014</item><item key="summary">ES cells express the miR-200 family which becomes down-regulated during the course of differentiation in serum.  We generated an ES cell line which expresses miR-200c and miR-141 upon addition of doxycycline. Microarrays were used to gain a global picture of differentiation when miR-200c and miR-141 expression were maintained throughout differentiation through the addition of doxycycline. A2.miR200c ES cells, which express miR-200c/miR-141 upon addition of doxycycline, were differentiated as embryoid bodies in six petri dishes.  Half of the samples were treated with doxycycline on days 2 and 4 of differentiation, and RNA was collected from all samples on days 3, 4, and 5.</item><item key="geo_gse_id">E-GEOD-24289</item><item key="sample_source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24289/samples/</item></data></biogps>
