{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM59472": {"TRANSFECTION": "Set8_deltaPIP2"}}, {"GSM594722": {"TRANSFECTION": "Wt-Set8b"}}, {"GSM594723": {"TRANSFECTION": "pMSCV-vector"}}], "id": 3810, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Ubiquitin-mediated proteolysis play a significant role in various biological processes including transcription, DNA repair and cell cycle...", "geo_gse_id": "E-GEOD-24170", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 3, "tags": ["cell", "histone", "osteosarcoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "stable-set8-effect-on-gene-expression-in-u2os-cell", "geo_id_plat": "E-GEOD-24170_A-AFFY-44", "name": "Stable Set8 effect on gene expression in U2OS cells", "created": "Sep.15, 2014", "summary": "Ubiquitin-mediated proteolysis play a significant role in various biological processes including transcription, DNA repair and cell cycle progression. The identification of Set8 and Set8b (a splice isoform) histone H4K20 methyl transferase as a substrate for the cullin-based ubiquitin ligase (CRL4-Cdt2) demonstrate that this pathway plays a significant role in promoting cell cycle progression, specifically promoting G2 progression. This study investigate the effect of failure to degrade Set8 in S-phase of the cell cycle via CRL4-Cdt2 on gene expression. We used microarrays to detail the effect of the expression of stable form of Set8b H4K20 mono-methyl transferase (Set8b_deltaPIP2) on gene expression Human osteosarcoma-derived human cells were transduced with retroviruses encoding either wt-Set8b or a mutant of Setb8 which is resistant to degradation via CRL4-Cdt2 ubiquitin ligase complex (Set8b_deltaPIP2) or with an a control pMSCV empty virus. 5 days after transduction, cells were harvested and the RNA was extracted by Trizol (Invitrogen) and hybridized to the affymetrix chips array.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24170", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24170/samples/"}