ArrayExpress Uploader0humanSet8_deltaPIP2Wt-Set8bpMSCV-vector3810arrayexpress_sid4Ubiquitin-mediated proteolysis play a significant role in various biological processes including transcription, DNA repair and cell cycle...E-GEOD-24170/profile/8773/arrayexpressuploader13cellhistoneosteosarcomaDec.12, 2014Falsestable-set8-effect-on-gene-expression-in-u2os-cellE-GEOD-24170_A-AFFY-44Stable Set8 effect on gene expression in U2OS cellsSep.15, 2014Ubiquitin-mediated proteolysis play a significant role in various biological processes including transcription, DNA repair and cell cycle progression. The identification of Set8 and Set8b (a splice isoform) histone H4K20 methyl transferase as a substrate for the cullin-based ubiquitin ligase (CRL4-Cdt2) demonstrate that this pathway plays a significant role in promoting cell cycle progression, specifically promoting G2 progression. This study investigate the effect of failure to degrade Set8 in S-phase of the cell cycle via CRL4-Cdt2 on gene expression. We used microarrays to detail the effect of the expression of stable form of Set8b H4K20 mono-methyl transferase (Set8b_deltaPIP2) on gene expression Human osteosarcoma-derived human cells were transduced with retroviruses encoding either wt-Set8b or a mutant of Setb8 which is resistant to degradation via CRL4-Cdt2 ubiquitin ligase complex (Set8b_deltaPIP2) or with an a control pMSCV empty virus. 5 days after transduction, cells were harvested and the RNA was extracted by Trizol (Invitrogen) and hybridized to the affymetrix chips array.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24170http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24170/samples/