ArrayExpress Uploader082630Kin-control0Kin-control0Kin-control0Kin-control0wild typecontrol0wild typecontrol0wild typecontrol0wild typecontrol24Kin-cAMP24Kin-cAMP24wild typecAMP24wild typecAMP24wild typecAMP24Kin-cAMP2Kin-cAMP2Kin-cAMP2Kin-cAMP2wild typecAMP2wild typecAMP2wild typecAMP6Kin-cAMP6Kin-cAMP6wild typecAMP6wild typecAMP6wild typecAMP6Kin-cAMParrayexpress_sid7Although a substantial number of hormones and drugs increase cellular cAMP levels, the global impact of cAMP and its major effector...15939874E-GEOD-2413/profile/8773/arrayexpressuploader326celllymphomaproteinDec.12, 2014Falsetranscription-profiling-by-array-of-mouse-s49-cellE-GEOD-2413_A-AFFY-23Transcription profiling by array of mouse S49 cells mutant for Kin after treatment with cAMPNov.23, 2014Although a substantial number of hormones and drugs increase cellular cAMP levels, the global impact of cAMP and its major effector mechanism, protein kinase A (PKA), on gene expression is not known. Here we show that treatment of wild-type S49 lymphoma cells for 24 h with 8-(4-chlorophenylthio)-cAMP (CPT-cAMP), a PKA-selective cAMP analog, alters the expression of ~4500 of ~13,600 unique genes. By contrast, gene expression was unaltered in Kin- S49 cells (that lack PKA) incubated with CPT-cAMP. Changes in mRNA and protein expression of several cell-cycle regulators accompanied cAMP-induced G1-phase cell-cycle arrest of wild-type S49 cells. Within 2 h, CPT-cAMP altered expression of 152 genes that contain evolutionarily conserved cAMP-response elements (CRE) within 5 kb of transcriptional start sites, including the circadian clock gene Per1. Thus, cAMP through its activation of PKA produces extensive transcriptional regulation in eukaryotic cells. These transcriptional networks include a primary group of CRE-containing genes and secondary networks that include the circadian clock.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-2413mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-2413/samples/