{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8263, "factors": [{"GSE2413GSM45464": {"time": 0, "genotype": "Kin-", "compound": "control"}}, {"GSE2413GSM45464": {"time": 0, "genotype": "Kin-", "compound": "control"}}, {"GSE2413GSM45464": {"time": 0, "genotype": "Kin-", "compound": "control"}}, {"GSE2413GSM45464": {"time": 0, "genotype": "Kin-", "compound": "control"}}, {"GSE2413GSM45451": {"time": 0, "genotype": "wild type", "compound": "control"}}, {"GSE2413GSM45451": {"time": 0, "genotype": "wild type", "compound": "control"}}, {"GSE2413GSM45451": {"time": 0, "genotype": "wild type", "compound": "control"}}, {"GSE2413GSM45451": {"time": 0, "genotype": "wild type", "compound": "control"}}, {"GSE2413GSM45476": {"time": 24, "genotype": "Kin-", "compound": "cAMP"}}, {"GSE2413GSM45476": {"time": 24, "genotype": "Kin-", "compound": "cAMP"}}, {"GSE2413GSM45461": {"time": 24, "genotype": "wild type", "compound": "cAMP"}}, {"GSE2413GSM45461": {"time": 24, "genotype": "wild type", "compound": "cAMP"}}, {"GSE2413GSM45461": {"time": 24, "genotype": "wild type", "compound": "cAMP"}}, {"GSE2413GSM45476": {"time": 24, "genotype": "Kin-", "compound": "cAMP"}}, {"GSE2413GSM45468": {"time": 2, "genotype": "Kin-", "compound": "cAMP"}}, {"GSE2413GSM45468": {"time": 2, "genotype": "Kin-", "compound": "cAMP"}}, {"GSE2413GSM45468": {"time": 2, "genotype": "Kin-", "compound": "cAMP"}}, {"GSE2413GSM45455": {"time": 2, "genotype": "wild type", "compound": "cAMP"}}, {"GSE2413GSM45455": {"time": 2, "genotype": "wild type", "compound": "cAMP"}}, {"GSE2413GSM45455": {"time": 2, "genotype": "wild type", "compound": "cAMP"}}, {"GSE2413GSM45472": {"time": 6, "genotype": "Kin-", "compound": "cAMP"}}, {"GSE2413GSM45472": {"time": 6, "genotype": "Kin-", "compound": "cAMP"}}, {"GSE2413GSM45458": {"time": 6, "genotype": "wild type", "compound": "cAMP"}}, {"GSE2413GSM45458": {"time": 6, "genotype": "wild type", "compound": "cAMP"}}, {"GSE2413GSM45458": {"time": 6, "genotype": "wild type", "compound": "cAMP"}}, {"GSE2413GSM45472": {"time": 6, "genotype": "Kin-", "compound": "cAMP"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 7, "summary_wrapped": "Although a substantial number of hormones and drugs increase cellular cAMP levels, the global impact of cAMP and its major effector...", "pubmed_id": 15939874, "geo_gse_id": "E-GEOD-2413", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 26, "tags": ["cell", "lymphoma", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-mouse-s49-cell", "geo_id_plat": "E-GEOD-2413_A-AFFY-23", "name": "Transcription profiling by array of mouse S49 cells mutant for Kin after treatment with cAMP", "created": "Nov.23, 2014", "summary": "Although a substantial number of hormones and drugs increase cellular cAMP levels, the global impact of cAMP and its major effector mechanism, protein kinase A (PKA), on gene expression is not known. Here we show that treatment of wild-type S49 lymphoma cells for 24 h with 8-(4-chlorophenylthio)-cAMP (CPT-cAMP), a PKA-selective cAMP analog, alters the expression of ~4500 of ~13,600 unique genes. By contrast, gene expression was unaltered in Kin- S49 cells (that lack PKA) incubated with CPT-cAMP. Changes in mRNA and protein expression of several cell-cycle regulators accompanied cAMP-induced G1-phase cell-cycle arrest of wild-type S49 cells. Within 2 h, CPT-cAMP altered expression of 152 genes that contain evolutionarily conserved cAMP-response elements (CRE) within 5 kb of transcriptional start sites, including the circadian clock gene Per1. Thus, cAMP through its activation of PKA produces extensive transcriptional regulation in eukaryotic cells. These transcriptional networks include a primary group of CRE-containing genes and secondary networks that include the circadian clock.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-2413", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-2413/samples/"}