{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3804, "factors": [{"GSM591694": {"TRANSFECTION": "vector"}}, {"GSM591694": {"TRANSFECTION": "vector"}}, {"GSM591694": {"TRANSFECTION": "vector"}}, {"GSM591697": {"TRANSFECTION": "KDM6B"}}, {"GSM591697": {"TRANSFECTION": "KDM6B"}}, {"GSM591697": {"TRANSFECTION": "KDM6B"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "In this study, we have investigated the effect of lysine-specific demethylase 6B (KDM6B) on gene expression in normal human germinal...", "pubmed_id": 21242977, "geo_gse_id": "E-GEOD-24044", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["lysine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "kdm6b-induced-gene-expression-changes-in-transfect", "geo_id_plat": "E-GEOD-24044_A-AFFY-44", "name": "KDM6B-induced gene expression changes in transfected CD10-positive GC B cells", "created": "Sep.15, 2014", "summary": "In this study, we have investigated the effect of lysine-specific demethylase 6B (KDM6B) on gene expression in normal human germinal center (GC) B cells using a non-viral vector-based system. Gene expression was compared between KDM6B-transfected and control vector-transfected GC B cells from three patients. RNA from the MACS-sorted transfected GC B cells was amplified. 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays. Differentially expressed genes were identified using Affymetrix GCOS pairwise analysis.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24044", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-24044/samples/"}