ArrayExpress Uploader0mouse7-12 weeksbone marrowCebpa Cre/wt R26 EYFP+7-12 weeksbone marrowCebpa Cre/wt R26 EYFP+7-12 weeksbone marrowCebpa Cre/wt R26 EYFP+7-12 weeksbone marrowCebpa Cre/wt R26 EYFP-7-12 weeksbone marrowCebpa Cre/wt R26 EYFP-E14.5fetal liverCebpa Cre/fl R26 EYFP+E14.5fetal liverCebpa Cre/fl R26 EYFP+E14.5fetal liverCebpa Cre/fl R26 EYFP-E14.5fetal liverCebpa Cre/fl R26 EYFP-E14.5fetal liverCebpa Cre/wt R26 EYFP+E14.5fetal liverCebpa Cre/wt R26 EYFP-6196arrayexpress_sid6C/EBPalpha is a transcription factor critically involved in myeloid development and indispensable for formation of granulocytes. To track...E-GEOD-23800/profile/8773/arrayexpressuploader411bonebone marrowliverDec.12, 2014Falseanalysis-of-differential-gene-expression-in-cebpaE-GEOD-23800_A-AFFY-45Analysis of differential gene expression in Cebpa-positive and Cebpa-negative hematopoietic stem cells using a Cebpa-Cre EYFP reporter mouse modelNov.11, 2014C/EBPalpha is a transcription factor critically involved in myeloid development and indispensable for formation of granulocytes. To track the cellular fate of stem and progenitor (LSK) cells, which express C/EBPalpha, we developed a mouse model expressing Cre recombinase from the Cebpa promoter and an inducible EYFP allele. We show that Cebpa/EYFP+ cells represent a significant subset of LSK cells, which predominantly give rise to myeloid cells in steady state hematopoiesis. C/EBPalpha induced a robust myeloid gene expression signature and downregulated E2A-induced regulators of early lymphoid development. In addition, Cebpa/EYFP+ cells comprise a fraction of early thymic progenitors (ETP) with robust myeloid potential. However, Cebpa/EYFP+ LSK and ETP cells retained the ability to develop into erythroid and T-lymphoid lineages, respectively. These findings support an instructive, but argue against a lineage restrictive role of C/EBPalpha in multipotent hematopoietic and thymic progenitors. We performed global gene expression profiling of double-sorted Cebpa/EYFP+ and Cebpa/EYFP- LSK cells of pooled Cebpa Cre/wt R26 EYFP reporter mice to identify differentially regulated genes in Cebpa+ versus Cebpa- LSK cells. RNA was isolated from three biological replicates of Cebpa/EYFP+ LSK cells and two biological replicates of Cebpa/EYFP- LSK cells. To determine if the identified genes were truly dependent on Cebpa expression, we also performed global gene expression profilling of Cebpa/EYFP+ and Cebpa/EYFP- fetal liver LSK cells of Cebpa Cre/fl R26 EYFP mice. Induction of Cebpa/Cre expression in these mice leads to Cre-mediated recombination of the floxed wt Cebpa allele resulting in a complete Cebpa knock-out. In this case, RNA was isolated from two biological replicates of either Cebpa/EYFP+ and Cebpa/EYFP- LSK cells. In addition, we included one biological replicate of Cebpa/EYFP+ and Cebpa/EYFP- fetal liver LSK cells of Cebpa Cre/wt R26 EYFP mice to determine the correlation of differentially regulated genes in bone marrow and fetal liver LSK cells.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23800http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23800/samples/