{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM587025": {"AGE": "7-12 weeks", "TISSUE": "bone marrow", "GENOTYPE": "Cebpa Cre/wt R26 EYFP", "CEBPA/EYFP INDUCTION": "+"}}, {"GSM587025": {"AGE": "7-12 weeks", "TISSUE": "bone marrow", "GENOTYPE": "Cebpa Cre/wt R26 EYFP", "CEBPA/EYFP INDUCTION": "+"}}, {"GSM587025": {"AGE": "7-12 weeks", "TISSUE": "bone marrow", "GENOTYPE": "Cebpa Cre/wt R26 EYFP", "CEBPA/EYFP INDUCTION": "+"}}, {"GSM587028": {"AGE": "7-12 weeks", "TISSUE": "bone marrow", "GENOTYPE": "Cebpa Cre/wt R26 EYFP", "CEBPA/EYFP INDUCTION": "-"}}, {"GSM587028": {"AGE": "7-12 weeks", "TISSUE": "bone marrow", "GENOTYPE": "Cebpa Cre/wt R26 EYFP", "CEBPA/EYFP INDUCTION": "-"}}, {"GSM587030": {"AGE": "E14.5", "TISSUE": "fetal liver", "GENOTYPE": "Cebpa Cre/fl R26 EYFP", "CEBPA/EYFP INDUCTION": "+"}}, {"GSM587030": {"AGE": "E14.5", "TISSUE": "fetal liver", "GENOTYPE": "Cebpa Cre/fl R26 EYFP", "CEBPA/EYFP INDUCTION": "+"}}, {"GSM587032": {"AGE": "E14.5", "TISSUE": "fetal liver", "GENOTYPE": "Cebpa Cre/fl R26 EYFP", "CEBPA/EYFP INDUCTION": "-"}}, {"GSM587032": {"AGE": "E14.5", "TISSUE": "fetal liver", "GENOTYPE": "Cebpa Cre/fl R26 EYFP", "CEBPA/EYFP INDUCTION": "-"}}, {"GSM587034": {"AGE": "E14.5", "TISSUE": "fetal liver", "GENOTYPE": "Cebpa Cre/wt R26 EYFP", "CEBPA/EYFP INDUCTION": "+"}}, {"GSM587035": {"AGE": "E14.5", "TISSUE": "fetal liver", "GENOTYPE": "Cebpa Cre/wt R26 EYFP", "CEBPA/EYFP INDUCTION": "-"}}], "id": 6196, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "C/EBPalpha is a transcription factor critically involved in myeloid development and indispensable for formation of granulocytes. To track...", "geo_gse_id": "E-GEOD-23800", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 11, "tags": ["bone", "bone marrow", "liver"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "analysis-of-differential-gene-expression-in-cebpa", "geo_id_plat": "E-GEOD-23800_A-AFFY-45", "name": "Analysis of differential gene expression in Cebpa-positive and Cebpa-negative hematopoietic stem cells using a Cebpa-Cre EYFP reporter mouse model", "created": "Nov.11, 2014", "summary": "C/EBPalpha is a transcription factor critically involved in myeloid development and indispensable for formation of granulocytes. To track the cellular fate of stem and progenitor (LSK) cells, which express C/EBPalpha, we developed a mouse model expressing Cre recombinase from the Cebpa promoter and an inducible EYFP allele. We show that Cebpa/EYFP+ cells represent a significant subset of LSK cells, which predominantly give rise to myeloid cells in steady state hematopoiesis. C/EBPalpha induced a robust myeloid gene expression signature and downregulated E2A-induced regulators of early lymphoid development. In addition, Cebpa/EYFP+ cells comprise a fraction of early thymic progenitors (ETP) with robust myeloid potential. However, Cebpa/EYFP+ LSK and ETP cells retained the ability to develop into erythroid and T-lymphoid lineages, respectively. These findings support an instructive, but argue against a lineage restrictive role of C/EBPalpha in multipotent hematopoietic and thymic progenitors. We performed global gene expression profiling of double-sorted Cebpa/EYFP+ and Cebpa/EYFP- LSK cells of pooled Cebpa Cre/wt R26 EYFP reporter mice to identify differentially regulated genes in Cebpa+ versus Cebpa- LSK cells. RNA was isolated from three biological replicates of Cebpa/EYFP+ LSK cells and two biological replicates of Cebpa/EYFP- LSK cells. To determine if the identified genes were truly dependent on Cebpa expression, we also performed global gene expression profilling of Cebpa/EYFP+ and Cebpa/EYFP- fetal liver LSK cells of Cebpa Cre/fl R26 EYFP mice. Induction of Cebpa/Cre expression in these mice leads to Cre-mediated recombination of the floxed wt Cebpa allele resulting in a complete Cebpa knock-out. In this case, RNA was isolated from two biological replicates of either Cebpa/EYFP+ and Cebpa/EYFP- LSK cells. In addition, we included one biological replicate of Cebpa/EYFP+ and Cebpa/EYFP- fetal liver LSK cells of Cebpa Cre/wt R26 EYFP mice to determine the correlation of differentially regulated genes in bone marrow and fetal liver LSK cells.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23800", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23800/samples/"}