{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 3777, "factors": [{"GSM579884": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "dH1F fibroblast"}}, {"GSM579884": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "dH1F fibroblast"}}, {"GSM579886": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "BJ fibroblast"}}, {"GSM579886": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "BJ fibroblast"}}, {"GSM579888": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "MRC5 fibroblast"}}, {"GSM579888": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "MRC5 fibroblast"}}, {"GSM579890": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "viral derived iPS", "CELL TYPE": "iPS cells derived from BJ fibroblast"}}, {"GSM57989": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "viral derived iPS", "CELL TYPE": "iPS cells derived from dH1F fibroblast"}}, {"GSM579892": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "viral derived iPS", "CELL TYPE": "iPS cells derived from dH1CF fibroblast"}}, {"GSM579892": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "viral derived iPS", "CELL TYPE": "iPS cells derived from dH1CF fibroblast"}}, {"GSM579892": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "viral derived iPS", "CELL TYPE": "iPS cells derived from dH1CF fibroblast"}}, {"GSM579892": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "viral derived iPS", "CELL TYPE": "iPS cells derived from dH1CF fibroblast"}}, {"GSM579896": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "viral derived iPS", "CELL TYPE": "iPS cells derived from MRC5 fibroblast"}}, {"GSM579896": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "viral derived iPS", "CELL TYPE": "iPS cells derived from MRC5 fibroblast"}}, {"GSM579898": {"CELL LINE": "H1 Human embryonic stem cells", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "not specified"}}, {"GSM579898": {"CELL LINE": "H1 Human embryonic stem cells", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "not specified"}}, {"GSM579900": {"CELL LINE": "H1 Human OGNES embryonic stem cells", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "not specified"}}, {"GSM57990": {"CELL LINE": "H9 Human embryonic stem cells", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "not specified"}}, {"GSM57990": {"CELL LINE": "H9 Human embryonic stem cells", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "not specified"}}, {"GSM579903": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from dH1F fibroblast"}}, {"GSM579903": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from dH1F fibroblast"}}, {"GSM579903": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from dH1F fibroblast"}}, {"GSM579903": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from dH1F fibroblast"}}, {"GSM579907": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from BJ fibroblast"}}, {"GSM579907": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from BJ fibroblast"}}, {"GSM579907": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from BJ fibroblast"}}, {"GSM579910": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from CF fibroblast"}}, {"GSM579910": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from CF fibroblast"}}, {"GSM579910": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from CF fibroblast"}}, {"GSM579913": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from MRC5 fibroblast"}}, {"GSM579913": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from MRC5 fibroblast"}}, {"GSM579913": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "RNA derived iPS", "CELL TYPE": "iPS cells derived from MRC5 fibroblast"}}, {"GSM579886": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "n/a", "CELL TYPE": "BJ fibroblast"}}, {"GSM579917": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "vehicle control", "CELL TYPE": "BJ fibroblast"}}, {"GSM579918": {"CELL LINE": "not specified", "REPROGRAMMING PROTOCOL": "modified-RNA (i.e., synthetic mRNA with GFP)", "CELL TYPE": "BJ fibroblast"}}], "pop_total": 0, "platform": 4, "summary_wrapped": "Clinical application of induced pluripotent stem (iPS) cells is limited by low efficiency of iPS derivation, and protocols that...", "geo_gse_id": "E-GEOD-23583", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 35, "tags": ["cell", "disease", "genome", "somatic cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "highly-efficient-reprogramming-to-pluripotency-and", "geo_id_plat": "E-GEOD-23583_A-AFFY-44", "name": "Highly efficient reprogramming to pluripotency and directed differentiation using synthetic mRNA", "created": "Sep.15, 2014", "summary": "Clinical application of induced pluripotent stem (iPS) cells is limited by low efficiency of iPS derivation, and protocols that permanently modify the genome to effect cellular reprogramming. Moreover, safe and effective means of directing the fate of patient-specific iPS cells towards clinically useful cell types are lacking. Here we describe a simple, non-mutagenic strategy for reprogramming cell fate based on administration of synthetic mRNA modified to overcome innate anti-viral responses. We show that this approach can reprogram multiple human cell types to pluripotency with efficiencies that greatly surpass established protocols. We further show that the same technology can be used to efficiently direct the differentiation of RNA-induced pluripotent stem (RiPS) cells into terminally differentiated myogenic cells. Our method represents a safe, efficient strategy for somatic cell reprogramming and directing cell fates that has broad applicability for basic research, disease modeling and regenerative medicine. We isolated RNA from human RNA derived iPS cells, viral derived iPS cells, different human fibroblasts and human embryonic stem cells for hybridization to the Affymetrix gene expression microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23583", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23583/samples/"}