ArrayExpress Uploader08498vehicle2 hoursvehicle2 hoursvehicle2 hoursaldosterone2 hoursaldosterone2 hoursaldosterone2 hoursvehicle4 hoursvehicle4 hoursvehicle4 hoursaldosterone4 hoursaldosterone4 hoursaldosterone4 hoursvehicle8 hoursvehicle8 hoursvehicle8 hoursaldosterone8 hoursaldosterone8 hoursaldosterone8 hoursarrayexpress_sid8The steroid hormone aldosterone plays a role in vascular function and disease. Aldosterone activates the mineralocorticoid receptor (MR),...20921624E-GEOD-23566/profile/8773/arrayexpressuploader218basaldiseasehormoneorgansteroid hormoneDec.12, 2014Falsealdosterone-regulated-expression-data-in-mouse-aorE-GEOD-23566_A-AFFY-36Aldosterone-Regulated Expression Data in Mouse AortaNov.24, 2014The steroid hormone aldosterone plays a role in vascular function and disease. Aldosterone activates the mineralocorticoid receptor (MR), a ligand-activated transcription factor. MR have been found to be expressed in vascular cells and vessels. We used microarrays to identify the global programme of gene expression changes in mouse aortas treated with vehicle (DMSO) or aldosterone. Wild type C57Bl6 male mice were treated with spironolactone (20 mg/kg/day, an MR antagonist) for 5 days by Sub-cutaneous pellet to suppress basal MR-mediated gene expression. Aortas were then harvested and treated ex vivo in organ culture with vehicle or 100 nM aldosterone for 2, 4, or 8 hours. Three aortas were pooled for each timepoint and treatment, and three pooled biological replicates were performed.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23566mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23566/samples/