{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6183, "factors": [{"GSM575743": {"TREATMENT": "No_Cytokine"}}, {"GSM575743": {"TREATMENT": "No_Cytokine"}}, {"GSM575745": {"TREATMENT": "IL6_IL1"}}, {"GSM575745": {"TREATMENT": "IL6_IL1"}}, {"GSM575747": {"TREATMENT": "IL6_IL1_IL23"}}, {"GSM575747": {"TREATMENT": "IL6_IL1_IL23"}}, {"GSM575749": {"TREATMENT": "IL6_IL1_TGFB"}}, {"GSM575749": {"TREATMENT": "IL6_IL1_TGFB"}}, {"GSM57575": {"TREATMENT": "IL6_TGFB_TGFBRi"}}, {"GSM575752": {"TREATMENT": "IL6_TGFB"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "CD4+ T cells that selectively produce interleukin (IL)-17, are critical for host defense and autoimmunity1-4. Crucial for T helper17...", "pubmed_id": 20962846, "geo_gse_id": "E-GEOD-23505", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 10, "tags": ["cell", "disease", "encephalomyelitis", "interleukin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "enhanced-pathogenicity-of-th17-cells-generated-in", "geo_id_plat": "E-GEOD-23505_A-AFFY-45", "name": "Enhanced Pathogenicity of Th17 cells Generated in the Absence of Transforming Growth Factor-\u03b2 Signaling", "created": "Nov.11, 2014", "summary": "CD4+ T cells that selectively produce interleukin (IL)-17, are critical for host defense and autoimmunity1-4. Crucial for T helper17 (Th17) cells in vivo5,6, IL-23 has been thought to be incapable of driving initial differentiation. Rather, IL-6 and transforming growth factor (TGF)-\u03b21 have been argued to be the factors responsible for initiating specification7-10. Herein, we show that Th17 differentiation occurs in the absence of TGF-\u03b2 signaling. Neither IL-6 nor IL-23 alone efficiently generated Th17 cells; however, these cytokines in combination with IL-1\u03b2 effectively induced IL-17 production in na\u00efve precursors, independently of TGF-\u03b2. Epigenetic modification of the Il17a/Il17f and Rorc promoters proceeded without TGF-\u03b21, allowing the generation of cells that co-expressed Ror\u03b3t and T-bet.           T-bet+Ror\u03b3t+ Th17 cells are generated in vivo during experimental allergic encephalomyelitis (EAE), and adoptively transferred Th17 cells generated with IL-23 in the absence of TGF-\u03b21 were more pathogenic in this experimental disease. These data suggest a new model for Th17 differentiation. Consistent with genetic data linking the IL23R with autoimmunity, our findings re-emphasize the role of IL-23 and therefore have important implications for the development of new therapies. Mouse T helper 17 cell differentiation with or without TGFB", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23505", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23505/samples/"}