ArrayExpress Uploader03765NoneNoneNoneLPS 6 hrLPS 6 hrLPS 6 hrIL10/dexamethasone 24 hrIL10/dexamethasone 24 hrIL10/dexamethasone 24 hrIL10/dexamethasone 24 hr, then LPS 6hrIL10/dexamethasone 24 hr, then LPS 6hrIL10/dexamethasone 24 hr, then LPS 6hrarrayexpress_sid4Little is known about the early transcriptional events in innate immune signaling in immature and tolerogenic monocyte-derived dendritic...21708028E-GEOD-23371/profile/8773/arrayexpressuploader112dendriticimmune systemmonocyteDec.12, 2014FalseE-GEOD-23371_A-AFFY-44transcriptomes-of-monocyte-derives-dcs-stimulatedTranscriptomes of monocyte-derives DCs stimulated with various compoundsSep.15, 2014Little is known about the early transcriptional events in innate immune signaling in immature and tolerogenic monocyte-derived dendritic cells (DCs), the professional antigen-presenting cells of our immune system. TLR ligands usually induce a proinflammatory transcriptional response, whereas IL10 and/or dexamethasone induce a more tolerogenic phenotype. We used Affymetrix microarrays to obtain detailed information underlying pro- and anti-inflammatory transcriptional responsesand transcriptional networks in DCs A pilot experiment was performed in which monocyte-derived DCs were either treated with TLR4 ligand LPS, or IL10 and dexamethason. Furthermore, IL10/dexamethason treated cells were also stimulated with LPS for an additional 6 hr. All samples were then subjected to global gene expression analysis using Affymetrix technology.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23371humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23371/samples/