ArrayExpress Uploaderarrayexpress_sid2358SU-DHL-1SU-DHL-1SU-DHL-1SU-DHL-1SU-DHL-1SU-DHL-1SU-DHL-1Ki-JKKi-JKKi-JKKi-JKKi-JKKi-JKKi-JK04C/EBPβ (CCAAT enhancer binding protein) is a transcription factor that plays a crucial role in survival and transformation of ALK+...23741337E-GEOD-23368/profile/8773/arrayexpressuploader114anaplastic large cell lymphomacellcentralchromatinlymphomaproteinDec.12, 2014FalseE-GEOD-23368_A-AFFY-44identification-of-cebp-target-genes-in-alk-anaplasIdentification of C/EBPβ Target Genes in ALK+ Anaplastic Large Cell Lymphoma (ALCL) by Gene Expression Profiling and Chromatin ImmunoprecipitationJul.12, 2014C/EBPβ (CCAAT enhancer binding protein) is a transcription factor that plays a crucial role in survival and transformation of ALK+ anaplastic large cell lymphoma (ALCL). The aim of this study was to identify the downstream targets of C/EBPβ responsible for ALK-mediated oncogenesis. C/EBPβ was knocked down in ALK+ ALCL cell lines with a C/EBPβ-shRNA, followed by gene expression profiling (GEP). GEP analysis revealed a reproducible signature of genes that were significantly regulated by C/EBPβ. Classification into biological categories revealed overrepresentation of genes involved in the immune response, apoptosis and cell proliferation. Transcriptional regulation by C/EBPβ was found in 6 of 11 (BCL2A1, G0S2, TRIB1, S100A9, DDX21 and DDIT4) genes investigated by chromatin immunoprecipitation. We demonstrated that BCL2A1, G0S2 and DDX21 play a crucial role in survival and proliferation of ALK+ ALCL cells. DDX21, a gene involved in rRNA biogenesis, was found differentially overexpressed in primary ALK+ ALCL cases. All three candidate genes were validated in primary ALCL cases by either immunohistochemistry or RT-qPCR. In conclusion, we identified and validated several key C/EBPβ-regulated genes with major impact on survival and cell growth in ALK+ ALCL, supporting the central role of C/EBPβ in ALK-mediated oncogenesis. Kijk and SUDHL1 cell lines transfected with shRNA for C/EBPbeta were compared to control cells (3 biological replicates per group) and untreated cells (1 biological replicate)http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23368humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23368/samples/