ArrayExpress Uploader06174MR fl / flNo treatmentMR fl / flNo treatmentMR fl / fl24 hours of 1 µM CorticosteroneMR fl / fl24 hours of 1 µM CorticosteroneMR fl / fl, LysM creNo treatmentMR fl / fl, LysM creNo treatmentMR fl / fl, LysM cre24 hours of 1 µM CorticosteroneMR fl / fl, LysM cre24 hours of 1 µM Corticosteronearrayexpress_sid6Inappropriate excess of the steroid hormone aldosterone, which is a mineralocorticoid receptor (MR) agonist, is associated with increased...20697155E-GEOD-23308/profile/8773/arrayexpressuploader28diseasehormonemacrophagesteroid hormoneDec.12, 2014Falseeffect-of-mineralocorticoid-receptor-deletion-on-gE-GEOD-23308_A-AFFY-45Effect of Mineralocorticoid Receptor deletion on glucocorticoid signalling in the macropahgeNov.11, 2014Inappropriate excess of the steroid hormone aldosterone, which is a mineralocorticoid receptor (MR) agonist, is associated with increased inflammation and risk of cardiovascular disease. MR antagonists are cardioprotective and antiinflammatory in vivo, and evidence suggests that they mediate these effects in part by aldosterone- independent mechanisms. We used affymetrix to characterize the effect of Mineralocorticoid Receptor deletion on macrophage transcriptional profile, and identify its requirement in normal glucocorticoid signalling. We isolated mouse peritoneal macrophages from Myeloid MRKO mice and Floxed Controls, cultured in the presence or absence of corticosterone. RNA was extracted for Affymetrix cDNA hybridization. Each sample was pooled from experiments performed in triplicate.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23308mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23308/samples/