{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM571000": {"DENDRITIC CELL": "CD8+CX3CR1+"}}, {"GSM571000": {"DENDRITIC CELL": "CD8+CX3CR1+"}}, {"GSM571002": {"DENDRITIC CELL": "CD4+"}}, {"GSM571002": {"DENDRITIC CELL": "CD4+"}}], "id": 6172, "pop_total": 0, "platform": 6, "summary_wrapped": "We describe a novel subset of CD8+ DCs in lymphoid organs of na\u00efve mice characterized by expression of the CX3CR1 chemokine receptor....", "pubmed_id": 20679228, "geo_gse_id": "E-GEOD-23212", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["chemokine", "genome", "immunoglobulin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-profiling-of-mouse-splenic-dendrit", "geo_id_plat": "E-GEOD-23212_A-AFFY-45", "name": "Gene expression profiling of mouse splenic Dendritic cells subsets", "created": "Nov.11, 2014", "summary": "We describe a novel subset of CD8+ DCs in lymphoid organs of na\u00efve mice characterized by expression of the CX3CR1 chemokine receptor. CX3CR1+CD8+ DCs lack hallmarks of classical CD8+ DCs, including IL12 secretion, the capacity to cross-present antigen and their developmental independence of the transcriptional factor BatF3. Gene expression profiling showed that CX3CR1+CD8+ DCs resemble CD8- cDCs. The microarray analysis further revealed a unique plasmacytoid DC (PDC) gene signature of CX3CR1+ CD8+ DCs. A PDC relationship of the cells is further supported by the fact that they harbor characteristic D-J immunoglobulin gene rearrangements and that development of CX3CR1+CD8+ DCs requires E2-2, the critical transcriptional regulator of PDCs. Thus, CX3CR1+ CD8+ DCs represent a unique DC subset, related to but distinct from PDCs. After collagenase D digestion, spleens from Cx3cr1gfp/+ C57BL/6 mice were enriched for CD11c+ cells by magnetic separation according to the manufacturer\u2019s protocol (MiltenyiBiotec GmbH). Splenic CD11chi cells were isolated using the FACS ARIA high-speed sorter (Becton-Dickinson). Total RNA was extracted and subjected to gene expression profiling using the Mouse Genome 430.2 Affymetrix GeneChip", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23212", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23212/samples/"}