ArrayExpress Uploaderarrayexpress_sidmouseC57BL/6B1aC57BL/6B1aC57BL/6B1aC57BL/6B1aB6.Sle2c1B1aB6.Sle2c1B1aB6.Sle2c1B1aB6.Sle2c1B1aC57BL/6BsC57BL/6BsC57BL/6BsC57BL/6BsB6.Sle2c1BsB6.Sle2c1BsB6.Sle2c1BsB6.Sle2c1Bs616506Sle2c1 is an NZM2410-derived lupus susceptibility locus that induces an expansion of the B1a cell compartment. B1a cells have a...E-GEOD-23114/profile/8773/arrayexpressuploader216cellcompartmentlupusperitoneal cavityDec.12, 2014Falsecell-cyclin-kinase-inhibitor-cdkn2c-regulates-b-ceE-GEOD-23114_A-AFFY-45Cell cyclin kinase inhibitor Cdkn2c regulates B cell homeostasis and function in the NZM2410-derived murine lupus susceptibility locus Sle2c1Nov.11, 2014Sle2c1 is an NZM2410-derived lupus susceptibility locus that induces an expansion of the B1a cell compartment. B1a cells have a repertoire enriched for autoreactivity, and an expansion of this B cell subset occurs in several mouse models of lupus. Here we showed that expression of Sle2c1 enhances NZB cellular phenotypes that have been associated with autoimmune pathogenesis. A combination of genetic mapping and candidate gene analysis presents Cdkn2c, a gene encoding for cyclin kinase inhibitor p18INK4c (p18), as the top candidate gene for inducing the Slec2c1 associated expansion of B1a cells. A novel SNP in the Cdkn2c promoter is associated with a significantly reduced Cdkn2c expression in the splenic B cells and B1a cells from Sle2c1-carrying mice, which leads to defective G1 cell cycle arrest in splenic B cells and increased proliferation of Pc B1a cells. As cell cycle is differentially regulated in B1a and B2 cells, these results suggest that Cdkn2c play a critical role in B1a cell self renewal, and that its impaired expression leads to an accumulation of these cells with high autoreactive potential. Total RNA from peritoneal cavity B cells (B1a) and splenic B cells (Bs) was isolated, with 4 biological replicates each. Gene expression data from C57BL/6 mice were compared with data from B6.Sle2c1 mice.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23114http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23114/samples/