{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM569443": {"STRAIN": "C57BL/6", "CELL TYPE": "B1a"}}, {"GSM569443": {"STRAIN": "C57BL/6", "CELL TYPE": "B1a"}}, {"GSM569443": {"STRAIN": "C57BL/6", "CELL TYPE": "B1a"}}, {"GSM569443": {"STRAIN": "C57BL/6", "CELL TYPE": "B1a"}}, {"GSM569447": {"STRAIN": "B6.Sle2c1", "CELL TYPE": "B1a"}}, {"GSM569447": {"STRAIN": "B6.Sle2c1", "CELL TYPE": "B1a"}}, {"GSM569447": {"STRAIN": "B6.Sle2c1", "CELL TYPE": "B1a"}}, {"GSM569447": {"STRAIN": "B6.Sle2c1", "CELL TYPE": "B1a"}}, {"GSM56945": {"STRAIN": "C57BL/6", "CELL TYPE": "Bs"}}, {"GSM56945": {"STRAIN": "C57BL/6", "CELL TYPE": "Bs"}}, {"GSM56945": {"STRAIN": "C57BL/6", "CELL TYPE": "Bs"}}, {"GSM56945": {"STRAIN": "C57BL/6", "CELL TYPE": "Bs"}}, {"GSM569455": {"STRAIN": "B6.Sle2c1", "CELL TYPE": "Bs"}}, {"GSM569455": {"STRAIN": "B6.Sle2c1", "CELL TYPE": "Bs"}}, {"GSM569455": {"STRAIN": "B6.Sle2c1", "CELL TYPE": "Bs"}}, {"GSM569455": {"STRAIN": "B6.Sle2c1", "CELL TYPE": "Bs"}}], "id": 6165, "pop_total": 0, "platform": 6, "summary_wrapped": "Sle2c1 is an NZM2410-derived lupus susceptibility locus that induces an expansion of the B1a cell compartment. B1a cells have a...", "geo_gse_id": "E-GEOD-23114", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 16, "tags": ["cell", "compartment", "lupus", "peritoneal cavity"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "cell-cyclin-kinase-inhibitor-cdkn2c-regulates-b-ce", "geo_id_plat": "E-GEOD-23114_A-AFFY-45", "name": "Cell cyclin kinase inhibitor Cdkn2c regulates B cell homeostasis and function in the NZM2410-derived murine lupus susceptibility locus Sle2c1", "created": "Nov.11, 2014", "summary": "Sle2c1 is an NZM2410-derived lupus susceptibility locus that induces an expansion of the B1a cell compartment. B1a cells have a repertoire enriched for autoreactivity, and an expansion of this B cell subset occurs in several mouse models of lupus. Here we showed that expression of Sle2c1 enhances NZB cellular phenotypes that have been associated with autoimmune pathogenesis. A combination of genetic mapping and candidate gene analysis presents Cdkn2c, a gene encoding for cyclin kinase inhibitor p18INK4c (p18), as the top candidate gene for inducing the Slec2c1 associated expansion of B1a cells.  A novel SNP in the Cdkn2c promoter is associated with a significantly reduced Cdkn2c expression in the splenic B cells and B1a cells from Sle2c1-carrying mice, which leads to defective G1 cell cycle arrest in splenic B cells and increased proliferation of Pc B1a cells.  As cell cycle is differentially regulated in B1a and B2 cells, these results suggest that Cdkn2c play a critical role in B1a cell self renewal, and that its impaired expression leads to an accumulation of these cells with high autoreactive potential. Total RNA from peritoneal cavity B cells (B1a) and splenic B cells (Bs) was isolated, with 4 biological replicates each. Gene expression data from C57BL/6 mice were compared with data from B6.Sle2c1 mice.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23114", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-23114/samples/"}