{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3744, "factors": [{"GSM565982": {"AGE": 25, "PROTOCOL": "control"}}, {"GSM565983": {"AGE": 30, "PROTOCOL": "control"}}, {"GSM565984": {"AGE": 25, "PROTOCOL": "treated with cytokines IL-2, IL-12 and IL-18"}}, {"GSM565985": {"AGE": 30, "PROTOCOL": "treated with cytokines IL-2, IL-12 and IL-18"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "We used Affymetrix expression arrays to determine changes in gene expression associated with activation of human NK cells mediated...", "pubmed_id": 20944005, "geo_gse_id": "E-GEOD-22919", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["cytokine", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcriptional-changes-in-il-2-il-12-and-il-18-st", "geo_id_plat": "E-GEOD-22919_A-AFFY-44", "name": "Transcriptional changes in IL-2, IL-12 and IL-18 stimulated human natural killer cells", "created": "Sep.15, 2014", "summary": "We used Affymetrix expression arrays to determine changes in gene expression associated with activation of human NK cells mediated through treatment with cytokines IL-2, IL-12 and IL-18 over a 24 hour period. Human natural killer cells were isolated via negative selection from PBMCs of healthy donors.  Cells were found to be > 95% CD3-CD56+.  RNA was harvested at time of isolation or after 24 hour stimulation from 8 x 10^6 cells per condition.  For stimulations, cells were incubated at 37C (5% CO2) in RPMI, suuplemented with 10% Fetal Bovine Serum at 1.5 x 10^6 cells/ml.  Cytokine stimualtions were conducted with IL-2 (100U/mL), IL-12 (10ng/mL) and IL-18 (100ng/mL) from 2 male donors (N = 4).  Expression analysis was carried out to determine transcriptional changes associated with 24 hr stimulation relative to freshly isolated cells.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22919", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22919/samples/"}