{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM563830": {"CELL LINE": "lung adenocarcinoma cell line CL1-5", "PROTOCOL": "control shRNA expression"}}, {"GSM56383": {"CELL LINE": "lung adenocarcinoma cell line CL1-5", "PROTOCOL": "G9a knockdown"}}, {"GSM563832": {"CELL LINE": "lung adenocarcinoma cell line CL1-0", "PROTOCOL": "control vector expression"}}, {"GSM563833": {"CELL LINE": "lung adenocarcinoma cell line CL1-0", "PROTOCOL": "G9a overexpression"}}], "id": 3736, "pop_total": 0, "platform": 4, "summary_wrapped": "G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regionsand is essential for murine embryogenesis . We...", "geo_gse_id": "E-GEOD-22810", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 4, "tags": ["adenocarcinoma", "lung", "lung adenocarcinoma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-22810_A-AFFY-44", "slug": "expression-data-from-g9a-overexpression-and-knockd", "geo_gds_id": "", "name": "Expression data from G9a overexpression and knockdown lung cancer cell lines", "created": "Sep.15, 2014", "summary": "G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regionsand is essential for murine embryogenesis . We demonstrate that G9a is endowed with methyltransferase activity to concomitantly repress the downstream effector Ep-CAM, thereby promoting the invasion step of the invasion-metastasis cascade. We used microarrays to detail the G9a regulated gene expression underlying invasion-metastasis cascade and identified distinct classes of up-regulated genes during this process. Lung adenocarcinoma cells with G9a knockdown or overexpression were selected for RNA extraction and hybridization on Affymetrix microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22810", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22810/samples/"}