BioGPS
  • Home
  • Help
  • Plugins
  • Datasets
  • Sign Up
  • Login
Examples: Gene Symbol(s), Gene Ontology, Splicing plugins, Melanoma datasets
advanced
Home › Dataset Library › Differential gene expression in SN12C.19 renal cells with insertion of the chromosomal 3p arm

Dataset: Differential gene expression in SN12C.19 renal cells with insertion of the chromosomal 3p arm

SN12C.19 is a renal carcinoma cell line with loss of heterozygosity in the 3p chromosomal region near the centromere. It is believed...

Registered by ArrayExpress Uploader
View Dataset

SN12C.19 is a renal carcinoma cell line with loss of heterozygosity in the 3p chromosomal region near the centromere. It is believed that a tumor suppressor gene is located in this region. In studies with athymic nude mice, tumor formation followed injection of SN12C.19 cells. Tumor formation did not follow by injection of the hybrid cell line SN19(3i)YY, which includes a very small fragment insertion of the 3p chromosomal arm. The goal of this array experiment was to identify genes differentially expressed in SN12C.19 by the introduction of a normal copy of the chromosomal 3p arm. SN12C.19 and SN19(3i)YY cells were hybridized to Affymetrix U133 Plus 2.0 Chips. Array quality was inspected with output from dChip software. Gene expression was quantified with PDNN software. There were 57 probesets showing differential expression on the 3p arm, corresponding to 46 genes. A suppression subtractive hybridization (SSH) library was previously constructed using as starting materials microcell hybrid clones which mapped the NRC-1 locus and containing defined fragments of chromosome 3p12 which were either suppressed or unsuppressed for tumorigencity following injection of microcell hybrid clones into athymic nude mice

Species:
human

Samples:
2

Source:
E-GEOD-22781

Updated:
Dec.12, 2014

Registered:
Sep.15, 2014


Factors: (via ArrayExpress)
Sample CELL LINE
GSM56332 SN12C19
GSM563322 SN19(3i)YY

Tags

  • arm
  • carcinoma
  • cell
  • centromere
  • chromosome
  • line
  • renal carcinoma

Other Formats

JSON    XML
  • About
  • Blog
  • Help
  • FAQ
  • Downloads
  • API
  • iPhone App
  • Email updates
© 2023 The Scripps Research Institute. All rights reserved. (ver 94eefe6 )
  • Terms of Use