{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 3733, "factors": [{"GSM56324": {"disease state": "epilepsy", "clinical treatment": "none", "time": 0}}, {"GSM563242": {"disease state": "epilepsy", "clinical treatment": "dexamethosone", "time": 2}}, {"GSM563243": {"disease state": "epilepsy", "clinical treatment": "dexamethosone", "time": 6}}, {"GSM563244": {"disease state": "epilepsy", "clinical treatment": "dexamethosone", "time": 24}}, {"GSM56324": {"disease state": "epilepsy", "clinical treatment": "none", "time": 0}}, {"GSM563242": {"disease state": "epilepsy", "clinical treatment": "dexamethosone", "time": 2}}, {"GSM563243": {"disease state": "epilepsy", "clinical treatment": "dexamethosone", "time": 6}}, {"GSM563244": {"disease state": "epilepsy", "clinical treatment": "dexamethosone", "time": 24}}, {"GSM56324": {"disease state": "epilepsy", "clinical treatment": "none", "time": 0}}, {"GSM563242": {"disease state": "epilepsy", "clinical treatment": "dexamethosone", "time": 2}}, {"GSM563243": {"disease state": "epilepsy", "clinical treatment": "dexamethosone", "time": 6}}, {"GSM563244": {"disease state": "epilepsy", "clinical treatment": "dexamethosone", "time": 24}}, {"GSM563253": {"disease state": "normal", "clinical treatment": "none", "time": 0}}, {"GSM563254": {"disease state": "normal", "clinical treatment": "dexamethosone", "time": 2}}, {"GSM563255": {"disease state": "normal", "clinical treatment": "dexamethosone", "time": 6}}, {"GSM563256": {"disease state": "normal", "clinical treatment": "dexamethosone", "time": 24}}], "pop_total": 0, "platform": 4, "summary_wrapped": "Article title: Expression, regulation and function of phosphofructo-kinase/fructose-biphosphatases (PFKFBs) in glucocorticoid-induced...", "pubmed_id": 21092265, "geo_gse_id": "E-GEOD-22779", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 16, "tags": ["acute lymphoblastic leukemia", "cell", "central", "epilepsy", "glucose", "leukemia", "lymphoblastic leukemia", "peripheral"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-22779_A-AFFY-44", "slug": "transcription-profiling-by-array-of-human-perip-13", "geo_gds_id": "", "name": "Transcription profiling by array of human peripheral blood mononuclear cells from patients with epilepsy over a time course of dexamethasone treatment", "created": "Sep.15, 2014", "summary": "Article title: Expression, regulation and function of phosphofructo-kinase/fructose-biphosphatases (PFKFBs) in glucocorticoid-induced apoptosis of acute lymphoblastic leukemia cells.  Glucocorticoids (GCs) cause apoptosis and cell cycle arrest in lymphoid cells and constitute a central component in the therapy of lymphoid malignancies, most notably childhood acute lymphoblastic leukemia (ALL). PFKFB2 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-2), a kinase controlling glucose metabolism, was identified by us previously as a GC response gene in expression profiling analyses performed in children with ALL during initial systemic GC mono-therapy. Since deregulation of glucose metabolism has been implicated in apoptosis induction, this gene and its relatives PFKFB1, 3, and 4 were further analyzed. Expression analyses in additional ALL children, non-leukemic individuals and leukemic cell lines confirmed frequent PFKFB2 induction by GC in most systems sensitive to GC-induced apoptosis, particularly in T-ALL cells. The 3 other family members, in contrast, were not or weakly expressed (PFKFB1 and 4) or not induced by GC (PFKFB3). Conditional PFKFB2 over-expression in the CCRF-CEM T-ALL in vitro model revealed that its 2 splice variants (15A and 15B) did not have any detectable effect on survival or cell cycle progression. Moreover, neither PFKFB2 splice variant significantly affected sensitivity to, or kinetics of, GC-induced apoptosis. Our data suggest that, at least in the model system investigated, PFKFB2 is not an essential upstream regulator of the anti-leukemic effects of GC. Gene expression profiles of 4 non-leukemic individuals (1 healthy and 3 with epilepsy) were generated from mononuclear cells isolated from peripheral blood samples before, and after 2, 6, and 24 hours of in-vivo glucocorticoid treatment.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22779", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22779/samples/"}