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<biogps><data><item key="owner">ArrayExpress Uploader</item><item key="pop_total">0</item><item key="species">human</item><item key="factors"><item><item key="GSM562105"><item key="GROWTH CONDITION">grown in oestrogen deprieved and tamoxifen containing media</item><item key="CELL LINE">T47D</item></item></item><item><item key="GSM562106"><item key="GROWTH CONDITION">grown in oestrogen deprieved and tamoxifen containing media</item><item key="CELL LINE">ZR75-1</item></item></item><item><item key="GSM562107"><item key="GROWTH CONDITION">grown in tamoxifen containing media</item><item key="CELL LINE">T47D</item></item></item><item><item key="GSM562108"><item key="GROWTH CONDITION">grown in tamoxifen containing media</item><item key="CELL LINE">ZR75-1</item></item></item></item><item key="id">3728</item><item key="ownerprofile_id">arrayexpress_sid</item><item key="platform">4</item><item key="summary_wrapped">Tamoxifen Resistant (TR) gene profile from Breast cancer cell lines T47D and ZR75-1 with their oestrogen-deprieved conterparts were...</item><item key="geo_gse_id">E-GEOD-22664</item><item key="owner_profile">/profile/8773/arrayexpressuploader</item><item key="factor_count">2</item><item key="sample_count">4</item><item key="tags"><item>breast</item><item>breast cancer</item><item>cancer</item><item>cell</item></item><item key="lastmodified">Dec.12, 2014</item><item key="is_default">False</item><item key="geo_gds_id"/><item key="slug">expression-data-from-tamoxifen-resistant-breast-ca</item><item key="geo_id_plat">E-GEOD-22664_A-AFFY-44</item><item key="name">Expression data from Tamoxifen resistant Breast Cancer Cell lines</item><item key="created">Sep.15, 2014</item><item key="summary">Tamoxifen Resistant (TR) gene profile from Breast cancer cell lines T47D and ZR75-1 with their oestrogen-deprieved conterparts were analysed for gene associated with TR. We used Microarray Affymetrix HU133plus 2.0 chips for gene expression of TR cell lines, normalised them against GEO data available for normal T47D (GSM70667) and ZR75-1 (GSM70668). We grew parental breast cancer cell lines in tamoxifen containing media (0.1 microM) for 6 months and labelled them tamoxifen resistant (TR). Oestrogen-Deprieved cells were grown in charcoal-stripped media for 6 months then tamoxifen (0.1 microM) was added to the media and cells maintained a further 6 months and termed Oestrogen deprieved-tamoxifen resistant (ODTR) .</item><item key="source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22664</item><item key="sample_source">http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22664/samples/</item></data></biogps>
