{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3723, "factors": [{"GSM560760": {"CELL TYPE": "PEO4 (ER+) ovarian cancer cells", "AGENT": "Estrogen"}}, {"GSM56076": {"CELL TYPE": "2008 (ER-) ovarian cancer cells", "AGENT": "Placebo"}}, {"GSM560762": {"CELL TYPE": "2008 (ER-) ovarian cancer cells", "AGENT": "Estrogen"}}, {"GSM560762": {"CELL TYPE": "2008 (ER-) ovarian cancer cells", "AGENT": "Estrogen"}}, {"GSM560760": {"CELL TYPE": "PEO4 (ER+) ovarian cancer cells", "AGENT": "Estrogen"}}, {"GSM560760": {"CELL TYPE": "PEO4 (ER+) ovarian cancer cells", "AGENT": "Estrogen"}}, {"GSM56076": {"CELL TYPE": "2008 (ER-) ovarian cancer cells", "AGENT": "Placebo"}}, {"GSM56076": {"CELL TYPE": "2008 (ER-) ovarian cancer cells", "AGENT": "Placebo"}}, {"GSM56076": {"CELL TYPE": "2008 (ER-) ovarian cancer cells", "AGENT": "Placebo"}}, {"GSM560762": {"CELL TYPE": "2008 (ER-) ovarian cancer cells", "AGENT": "Estrogen"}}, {"GSM560762": {"CELL TYPE": "2008 (ER-) ovarian cancer cells", "AGENT": "Estrogen"}}, {"GSM56077": {"CELL TYPE": "PEO4 (ER+) ovarian cancer cells", "AGENT": "Placebo"}}, {"GSM560760": {"CELL TYPE": "PEO4 (ER+) ovarian cancer cells", "AGENT": "Estrogen"}}, {"GSM56077": {"CELL TYPE": "PEO4 (ER+) ovarian cancer cells", "AGENT": "Placebo"}}, {"GSM56077": {"CELL TYPE": "PEO4 (ER+) ovarian cancer cells", "AGENT": "Placebo"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Menopausal estrogen (E2) replacement therapy increases the risk of estrogen receptor (ER)-positive epithelial ovarian cancers (EOC)....", "pubmed_id": 20959477, "geo_gse_id": "E-GEOD-22600", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 15, "tags": ["breast", "breast cancer", "cancer", "cell", "disease", "estrogen", "hormone", "lymph", "lymph node"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "tissue-specific-pathways-for-estrogen-regulation-o", "geo_id_plat": "E-GEOD-22600_A-AFFY-44", "name": "Tissue Specific Pathways for Estrogen Regulation of Ovarian Cancer Growth and Metastasis", "created": "Sep.15, 2014", "summary": "Menopausal estrogen (E2) replacement therapy increases the risk of estrogen receptor (ER)-positive epithelial ovarian cancers (EOC). Whether E2 is tumorigenic or promotes expansion of undiagnosed pre-existing disease is unknown. To determine E2 effects on tumor promotion, we developed an intraperitoneal mouse xenograft model using ZsGreen fluorescent ER- 2008 and ER+ PEO4 human EOC cells. Tumor growth was quantified by in vivo fluorescent imaging. In ER+ tumors, E2 significantly increased size, induced progesterone receptors, and promoted lymph node metastasis, confirming that ER are functional and foster aggressiveness. Laser captured human EOC cells from ER- and ER+ xenografted tumors were profiled for expression of E2-regulated genes. Three classes of E-regulated EOC genes were defined, but less than 10% were shared with E-regulated breast cancer genes. Since breast cancer selective ER modulators (SERM) are therapeutically ineffective in EOC, we suggest that our EOC-specific E-regulated genes can assist pharmacologic discovery of ovarian targeted SERM. 15 samples were included in this experiment with a 2x2 factorial design with 2 different cell lines (2008 and PEO4) and 2 different hormone treatments (E for Estrogen and C for Placebo Control) and 4 replicates per treatment. 1 sample was excluded (a replicate of PEO4 with C treatment) because of poor quality.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22600", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22600/samples/"}