{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3720, "factors": [{"GSM564444": {"CELL TYPE": "colon cancer cell line"}}, {"GSM564444": {"CELL TYPE": "colon cancer cell line"}}, {"GSM564444": {"CELL TYPE": "colon cancer cell line"}}, {"GSM564444": {"CELL TYPE": "colon cancer cell line"}}, {"GSM564444": {"CELL TYPE": "colon cancer cell line"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Surprisingly few pathways signal between cells, raising questions about mechanisms for tissue-specific responses. In particular, Wnt...", "pubmed_id": 20696899, "geo_gse_id": "E-GEOD-22572", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 5, "tags": ["cancer", "cell", "chromatin", "colon", "colon cancer", "colorectal cancer", "genome", "intestinal epithelium", "intestine", "line", "nucleotide"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "tcf4-and-cdx2-major-transcription-factors-for-inte", "geo_id_plat": "E-GEOD-22572_A-AFFY-44", "name": "TCF4 and CDX2, major transcription factors for intestinal function, converge on the same cis-regulatory regions", "created": "Sep.15, 2014", "summary": "Surprisingly few pathways signal between cells, raising questions about mechanisms for tissue-specific responses. In particular, Wnt ligands signal in many mammalian tissues, including the intestinal epithelium, where constitutive signaling causes cancer. Genome-wide analysis of DNA cis-regulatory regions bound by the intestine-restricted transcription factor CDX2 in colonic cells uncovered highly significant over-representation of sequences that bind TCF4, a transcriptional effector of intestinal Wnt signaling. Chromatin immunoprecipitation confirmed TCF4 occupancy at most such sites and co-occupancy of CDX2 and TCF4 across short distances. A region spanning the single nucleotide polymorphism rs6983267, which lies within a MYC enhancer and confers colorectal cancer risk in humans, represented one of many co-occupied sites. Co-occupancy correlated with intestine-specific gene expression and CDX2 loss reduced TCF4 binding.These results implicate CDX2 in directing TCF4 binding in intestinal cells. Co-occupancy of regulatory regions by signal-effector and tissue-restricted transcription factors may represent a general mechanism for ubiquitous signaling pathways to achieve tissue-specific outcomes. A series of ChIP-chip experiments identified the CDX2 cistrome and discovered and validated extensive co-binding with TCF4 in colon cancer cell lines  Transcriptional profiling following shRNA-mediated CDX2 knockdown was employed to identify CDX2-dependent gene expression in the human colon cancer cell line Caco2", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22572", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22572/samples/"}